中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1779-1783.doi: 10.4103/1673-5374.194722

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Protective effect of ginkgo proanthocyanidins against cerebral ischemia/reperfusion injury associated with its antioxidant effects

  

  • 收稿日期:2016-08-24 出版日期:2016-11-30 发布日期:2016-11-30

Protective effect of ginkgo proanthocyanidins against cerebral ischemia/reperfusion injury associated with its antioxidant effects

Wang-li Cao1, Hai-bo Huang2, Ling Fang2, 3, Jiang-ning Hu1, 3, Zhu-ming Jin2, Ru-wei Wang1, 2, 3, *   

  1. 1 Zhejiang Modern Chinese Medicine and Natural Drug Research Academy Co., Ltd., Hangzhou, Zhejiang Province, China 2 Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou, Zhejiang Province, China 3 Zhejiang Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou, Zhejiang Province, China
  • Received:2016-08-24 Online:2016-11-30 Published:2016-11-30
  • Contact: Ru-wei Wang, Ph.D., wangrw@conbagroup.com.

摘要:

原花青素已被证实能有效保护脑缺血神经元,但机制尚待进一步探讨。实验在大鼠脑缺血再灌注前72,48,24及1h时腹腔注射20,40,80 mg/kg银杏原花青素,见银杏原花青素能有效减轻其神经功能损伤,缩小梗死体积,提升脑组织中超氧化物岐化酶水平,降低丙二醛及一氧化氮水平,效果与治疗脑缺血再灌注损伤的阳性药物尼莫地平接近;同时以葡萄籽原花青素(40 mg/kg)的干预实验证实,其他来源的原花青素也有类似效果,说明银杏原花青素可有效减轻脑缺血再灌注损伤,保护缺血脑组织,其效果应与抗氧化作用有关。 

orcid: 0000-0002-4354-250X (Ru-wei Wang)

关键词: 神经再生, 局灶性脑缺血再灌注, Ephrin-B2, 血管生成, 血管生成素1, 血管生成素2, 神经保护

Abstract:

Proanthocyanidins have been shown to effectively protect ischemic neurons, but its mechanism remains poorly understood. Ginkgo proanthocyanidins (20, 40, 80 mg/kg) were intraperitoneally administered 1, 24, 48 and 72 hours before reperfusion. Results showed that ginkgo proanthocyanidins could effectively mitigate neurological disorders, shorten infarct volume, increase superoxide dismutase activity, and decrease malondialdehyde and nitric oxide contents. Simultaneously, the study on grape seed proanthocyanidins (40 mg/kg) confrmed that different sources of proanthocyanidins have a similar effect. Te neurological outcomes of ginkgo proanthocyanidins were similar to that of nimodipine in the treatment of cerebral ischemia/reperfusion injury. Our results suggest that ginkgo proanthocyanidins can effectively lessen cerebral ischemia/reperfusion injury and protect ischemic brain tissue and these effects are associated with antioxidant properties.

Key words: nerve regeneration, cerebral ischemia/reperfusion injury, proanthocyanidins, nimodipine, superoxide dismutase, malondialdehyde, nitric oxide, neural regeneration