中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1784-1789.doi: 10.4103/1673-5374.194723

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Ephrin-B2影响了局灶性脑缺血再灌注后血管生成素的表达

  

  • 收稿日期:2016-10-10 出版日期:2016-11-30 发布日期:2016-11-30

Effect of ephrin-B2 on the expressions of angiopoietin-1 and -2 afer focal cerebral ischemia/reperfusion

Hui Xiao1, Qing Huang2, Jia-qi Wang2, Qing-qing Deng2, Wen-ping Gu2, *   

  1. 1 Department of Neurology, Changsha Municipal Central Hospital, Changsha, Hunan Province, China 2 Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China
  • Received:2016-10-10 Online:2016-11-30 Published:2016-11-30
  • Contact: Wen-ping Gu, M.D., guwenping393@sohu.com.

摘要:

研究显示EphrinB2可参与血管再生过程,但具体机制尚不明确。实验以局灶性大脑中动脉闭塞的方法制备局灶性脑缺血再灌注大鼠模型,再灌注24h后连续3d通过微渗透泵向侧脑室注射10μg EphrinB2镶合蛋白。以Western blot检测和实时定量反转录PCR检测可见经EphrinB2治疗的局灶性脑缺血再灌注大鼠损伤侧半暗带中血管生成素1 mRNA及蛋白表达在再灌注后第4天下降,在28d时上升,而血管生成素2 mRNA及蛋白水平则一直保持较高水平。荧光双染中可见血管生成素1和血管生成素2在主要表达于CD31阳性的血管内皮细胞共表达。结果说明Ephrin-B2影响了局灶性脑缺血再灌注后血管生成素的表达。

orcid: 0000-0002-5018-4886 (Wen-ping Gu)

关键词: 神经再生, 局灶性脑缺血再灌注, Ephrin-B2, 血管生成, 血管生成素1, 血管生成素2, 神经保护

Abstract:

Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Ten, ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 (Ang-1) mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 afer reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 (Ang-2) were highly up-regulated at all time points tested. Double immuno?uorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. Tese fndings indicate that ephrin-B2 in?uences the expressions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.

Key words: nerve regeneration, focal cerebral ischemia/reperfusion, ephrin-B2, angiogenesis, angiopoietin-1, angiopoietin-2, neuroprotection