中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1810-1815.doi: 10.4103/1673-5374.194751

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

三维管状胶原基质模型能更好反映哺乳动物脊髓损伤的多种变化

  

  • 出版日期:2016-11-30 发布日期:2016-11-30

Development of a 3D matrix for modeling mammalian spinal cord injury in vitro

Juan Felipe Diaz Quiroz1, 2, #, Yuping Li3, #, Conrado Aparicio3, Karen Echeverri1, *   

  1. 1 Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA 2 Current address: Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA 3 Minnesota Dental Research Center for Biomaterials and Biomechanics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, USA
  • Online:2016-11-30 Published:2016-11-30
  • Contact: Karen Echeverri, Ph.D., echev020@umn.edu.

摘要:

为研究脊髓损伤复杂的分子和细胞学机制,最常见的是脊髓挫伤或横断伤大鼠和小鼠模型,但由于损伤脊髓的内环境的复杂,细胞间的相互作用不十分明了,因而科学家们仍希望找到令人满意的可反映损伤脊髓内部环境变化的模型。实验由此建立了一个三维管状的胶原基质模型,其结构与脊髓中央管十分相似。结果显示,星形胶质细胞在这个胶原基质模型上生长良好,但当受到压迫时,细胞的反应与体内反应一致,均会出现反应性胶质增生,这也是导致胶质瘢痕形成经历的过程,是导致脊髓损伤再生修复受阻的因素。实验建立的三维基质模型将有希望用于影响脊髓损伤后胶质瘢痕形成的药物研究。 

orcid: 0000-0002-4658-8095 (Karen Echeverri)

关键词: 神经再生, 脊髓损伤, 胶质细胞增生, 星形胶质细胞, 胶质瘢痕, 体外模型, 基质, 胶原

Abstract: Spinal cord injury affects millions of people around the world, however, limited therapies are available to improve the quality of life of these patients. Spinal cord injury is usually modeled in rats and mice using contusion or complete transection models and this has led to a deeper understanding of the molecular and cellular complexities of the injury. However, it has not to date led to development of successful novel therapies, this is in part due to the complexity of the injury and the difculty of deciphering the exact roles and interactions of different cells within this complex environment. Here we developed a collagen matrix that can be molded into the 3D tubular shape with a lumen and can hence support cell interactions in a similar architecture to a spinal cord. We show that astrocytes can be successfully grown on this matrix in vitro and when injured, the cells respond as they do in vivo and undergo reactive gliosis, one of the steps that lead to formation of a glial scar, the main barrier to spinal cord regeneration. In the future, this system can be used to quickly assess the effect of drugs on glial scar protein activity or to perform live imaging of labeled cells afer exposure to drugs.

Key words: nerve regeneration, spinal cord injury, 3D scaffold, in vitro models, astrocytes, reactive gliosis, neural regeneration