中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (2): 235-241.doi: 10.4103/1673-5374.200807

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

3'-大豆苷元磺酸钠可以改善脑缺血再灌注损伤线粒体的功能

  

  • 收稿日期:2017-01-19 出版日期:2017-02-15 发布日期:2017-02-15
  • 基金资助:

    中国国家自然科学基金项目(81160399,81560583),中国科研落地项目(KJLD13085),江西省教育厅科技项目(GJJ12560)

3′-Daidzein sulfonate sodium improves mitochondrial functions after cerebral ischemia/reperfusion injury

Wa Yuan, Qin Chen, Jing Zeng, Hai Xiao, Zhi-hua Huang, Xiao Li, Qiong Lei   

  1. Gannan Medical University, Ganzhou, Jiangxi Province, China
  • Received:2017-01-19 Online:2017-02-15 Published:2017-02-15
  • Contact: Jing Zeng,zengjing6168@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81160399, 81560583; the Science and Technology Landing Project of China, No. KJLD13085; the Science and Technology Project of the Education Department of Jiangxi Province of China, No. GJJ12560.

摘要:

3'-大豆苷元磺酸钠是一种由葛根有效成分黄豆苷原新合成的水溶性化合物,对脑缺血再灌注损伤有保护作用,作者希望阐明其作用机制。实验设计在大鼠脑缺血再灌注损伤后10min,8,16h时尾静脉注射3'-大豆苷元磺酸钠,发现模型大鼠大脑线粒体肿胀明显减轻,线粒体膜电位提升,线粒体超氧化物歧化酶、谷胱甘肽过氧化物酶活性增加,丙二醛含量降低,血脑屏障结构完整性提高,通透性降低。说明3'-大豆苷元磺酸钠改善脑能量代谢、抗氧化和维持血脑屏障完整性的作用,产生脑缺血再灌注损伤后线粒体功能的保护效应。

ORCID:0000-0002-1265-1180(Jing Zeng)

关键词: 神经再生, 3'-大豆苷元磺酸钠, 脑缺血再灌注损伤, 脑梗死体积, 抗氧化, 线粒体, 线粒体膜肿胀, 线粒体膜电位, 超氧化物歧化酶, 丙二醛, 谷胱甘肽过氧化物酶, 血脑屏障

Abstract:

3′-Daidzein sulfonate sodium is a new synthetic water-soluble compound derived from daidzein (an active ingredient of the kudzu vine root). It has been shown to have a protective effect on cerebral ischemia/reperfusion injury in rats. We plan to study the mechanism of its protective effect. 3′-Daidzein sulfonate sodium was injected in rats after cerebral ischemia/reperfusion injury. Results showed that 3′-daidzein sulfonate sodium significantly reduced mitochondrial swelling, significantly elevated the mitochondrial membrane potential, increased mitochondrial superoxide dismutase and glutathione peroxidase activities, and decreased mitochondrial malondialdehyde levels. 3′-Daidzein sulfonate sodium improved the structural integrity of the blood-brain barrier and reduced blood-brain barrier permeability. These findings confirmed that 3′-daidzein sulfonate sodium has a protective effect on mitochondrial functions after cerebral ischemia/reperfusion injury, improves brain energy metabolism, and provides protection against blood-brain barrier damage.

Key words: nerve regeneration, 3′-daidzein sulfonate sodium, cerebral ischemia/reperfusion injury, infarct volume, anti-oxidation, mitochondria, mitochondrial membrane swelling, mitochondrial membrane potential, superoxide dismutase, malondialdehyde, glutathione peroxidase, blood-brain barrier, neural regeneration