中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (5): 757-760.doi: 10.4103/1673-5374.206645

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

聚乙二醇促进胼胝体横断后轴突传导的恢复

  

  • 收稿日期:2017-05-11 出版日期:2017-05-15 发布日期:2017-05-15

Polyethylene glycol restores axonal conduction after corpus callosum transection

Ravinder Bamba1, 2, D. Colton Riley1, 3, Richard B. Boyer1, Alonda C. Pollins1, R. Bruce Shack1, Wesley P. Thayer1   

  1. 1 Department of Plastic Surgery, Vanderbilt University Medical Center, Nashville, TN, USA; 2 Department of Surgery, Georgetown University, Washington, DC, USA; 3 Georgetown University School of Medicine, Washington, DC, USA
  • Received:2017-05-11 Online:2017-05-15 Published:2017-05-15
  • Contact: Ravinder Bamba, M.D., rbamba@gmail.com.

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摘要：

鉴于聚乙二醇（PEG）可恢复动物模型周围神经横断后的轴突连续性的以往研究成果，实验假设PEG还可以恢复中枢神经系统的轴突连续性，为验证该假设，实验在麻醉后处死SD大鼠，制备脑冠状切片。使用3Brain高分辨率微电极阵列（MEA）检测离体脑片的胼胝体的平均放电频率（MFR）和峰值振幅，随后横断胼胝体，将胼胝体断端孵育于聚乙二醇溶液中。MEA检测显示PEG能够恢复中枢神经横断后的电生理信号。应用聚乙二醇后，损伤部位的平均放电频率和峰值振幅出现许多尖峰。中线和非中线间胼胝体的平均放电频率和峰值振幅差异明显，说明聚乙二醇可恢复中枢神经横断后的传导和轴突融合。

ORCID：0000-0002-5432-2764(Ravinder Bamba)

关键词: 神经再生, 中枢神经损伤, 聚乙二醇, 神经修复, 轴突融合, 胼胝体, 轴突传导

Abstract:

Polyethylene glycol (PEG) has been shown to restore axonal continuity after peripheral nerve transection in animal models. We hypothesized that PEG can also restore axonal continuity in the central nervous system. In this current experiment, coronal sectioning of the brains of Sprague-Dawley rats was performed after animal sacrifice. 3Brain high-resolution microelectrode arrays (MEA) were used to measure mean firing rate (MFR) and peak amplitude across the corpus callosum of the ex-vivo brain slices. The corpus callosum was subsequently transected and repeated measurements were performed. The cut ends of the corpus callosum were still apposite at this time. A PEG solution was applied to the injury site and repeated measurements were performed. MEA measurements showed that PEG was capable of restoring electrophysiology signaling after transection of central nerves. Before injury, the average MFRs at the ipsilateral, midline, and contralateral corpus callosum were 0.76, 0.66, and 0.65 spikes/second, respectively, and the average peak amplitudes were 69.79, 58.68, and 49.60 μV, respectively. After injury, the average MFRs were 0.71, 0.14, and 0.25 spikes/second, respectively and peak amplitudes were 52.11, 8.98, and 16.09 μV, respectively. After application of PEG, there were spikes in MFR and peak amplitude at the injury site and contralaterally. The average MFRs were 0.75, 0.55, and 0.47 spikes/second at the ipsilateral, midline, and contralateral corpus callosum, respectively and peak amplitudes were 59.44, 45.33, 40.02 μV, respectively. There were statistically differences in the average MFRs and peak amplitudes between the midline and non-midline corpus callosum groups (P < 0.01, P < 0.05). These findings suggest that PEG restores axonal conduction between severed central nerves, potentially representing axonal fusion.

Key words: nerve regeneration, polyethylene glycol, nerve repair, axonal fusion, central nerve injury, axonal conduction, corpus callosum, neural regeneration