中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (5): 804-811.doi: 10.4103/1673-5374.206652

• 原著:颅神经损伤修复保护与再生 • 上一篇    下一篇

电针风池穴抑制偏头痛大鼠三叉神经血管系统三级神经元中降钙素基因相关肽水平

  

  • 收稿日期:2017-04-25 出版日期:2017-05-15 发布日期:2017-05-15
  • 基金资助:

    国家自然科学基金项目（81603683）; 国家基础研究项目（2014CB543203）; 北京市科委项目（Z171100001017033）

Electroacupuncture at Fengchi (GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine

Luo-peng Zhao1, 2, Lu Liu1, Pei Pei3, Zheng-yang Qu1, Yu-pu Zhu1, 2, Lin-peng Wang1   

  1. 1 Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China; 2 Beijing University of Chinese Medicine, Beijing, China; 3 The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui Province, China
  • Received:2017-04-25 Online:2017-05-15 Published:2017-05-15
  • Contact: Lin-peng Wang,wlp5558@sina.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81603683; a grant from the National Basic Research Program of China, No. 2014CB543203; a grant from the Beijing Municipal Science & Technology Commission of China, No. Z171100001017033.

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摘要：

大多数偏头痛患者伴有皮肤异常性疼痛，其潜在机制尚不清楚。由于降钙素基因相关肽在偏头痛病理生理过程中起重要作用，可能是电针治疗偏头痛及其相关皮肤异常性疼痛的一个潜在靶点。为此，实验通过重复电刺激上矢状窦建立偏头痛大鼠模型，对双侧风池穴进行电针治疗，刺激深度2-3mm，频率2/15Hz，强度0.5-1.0mA，15min/d，连续7d。显示大鼠头面及足底疼痛阈值提升，三叉神经血管系统中的三叉神经节、三叉神经尾侧核和丘脑腹后内侧核中的降钙素基因相关肽蛋白表达明显下降，三叉神经尾侧核和丘脑腹后内侧核中未见降钙素基因相关肽免疫阳性细胞。结果提示电针可通过抑制调节三叉神经血管系统中调节降钙素基因相关肽的表达，缓解偏头痛及其相关的皮肤异常性疼痛。

ORCID：0000-0002-0775-9939(Lin-peng Wang)

关键词: 神经再生, 电针, 偏头痛, 降钙素基因相关肽, 皮肤异常性疼痛, 镇痛, 风池穴, 三叉神经节

Abstract:

Most migraine patients suffer from cutaneous allodynia; however, the underlying mechanisms are unclear. Calcitonin gene-related peptide (CGRP) plays an important role in the pathophysiology of migraine, and it is therefore, a potential therapeutic target for treating the pain. In the present study, a rat model of conscious migraine, induced by repeated electrical stimulation of the superior sagittal sinus, was established and treated with electroacupuncture at Fengchi (GB20) (depth of 2–3 mm, frequency of 2/15 Hz, intensity of 0.5–1.0 mA, 15 minutes/day, for 7 consecutive days). Electroacupuncture at GB20 significantly alleviated the decrease in hind paw and facial withdrawal thresholds and significantly lessened the increase in the levels of CGRP in the trigeminal ganglion, trigeminal nucleus caudalis and ventroposterior medial thalamic nucleus in rats with migraine. No CGRP-positive cells were detected in the trigeminal nucleus caudalis or ventroposterior medial thalamic nucleus by immunofluorescence. Our findings suggest that electroacupuncture treatment ameliorates migraine pain and associated cutaneous allodynia by modulating the trigeminovascular system ascending pathway, at least in part by inhibiting CGRP expression in the trigeminal ganglion.

Key words: nerve regeneration, electroacupuncture, migraine, calcitonin gene-related peptide, cutaneous allodynia, anti-nociceptive, Fengchi, trigeminal ganglion, neural regeneration