中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (1): 156-171.doi: 10.4103/1673-5374.243721

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

单侧眼损伤后虹鳟脑内视神经Neurolin的表达和Pax6阳性细胞巢的激活

  

  • 出版日期:2019-01-15 发布日期:2019-01-15
  • 基金资助:

    这项工作得到了俄罗斯联邦总统（MD-4318.2015.4）和俄罗斯科学院远东分部2015-2017基础研究计划的支持

Neurolin expression in the optic nerve and immunoreactivity of Pax6-positive niches in the brain of rainbow trout (Oncorhynchus mykiss) after unilateral eye injury

Evgeniya V. Pushchina1, 2, Anatoly A. Varaksin1   

  1. 1 National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia
    2 A.A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine
  • Online:2019-01-15 Published:2019-01-15
  • Contact: Evgeniya V. Pushchina, DSci, PhD, puschina@mail.ru.
  • Supported by:

    This work was supported by the President of the Russian Federation (grant No. MD-4318.2015.4; to EVP) and by the Far East Branch of the Russian Academy of Sciences within the Program for Basic Research for 2015–2017 (grant No. 15-I-6-116; section III ;  to AAV  and EVP).

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摘要：

与哺乳动物中的星形胶质细胞相反，鱼类星形胶质细胞在脑损伤后可促进轴突再生并积极参与再生过程， Zn-8标记的蛋白质Neurolin参与修复金鱼受损的视神经。实验在虹鳟单侧眼机械损伤后1周，检测虹鳟（Oncorhynchus mykiss）视神经和视交叉Zn-8表达及各脑区中影响神经系统发育的Pax6的表达。在免疫组化染色后见，损伤侧视神经乳头和眶内段视神经Zn8阳性细胞数明显升高；损伤侧视神经近端和远端也呈现此种变化。从而表明，Zn8阳性星形胶质细胞参与视神经再生过程。经ELISA检测显示，Pax6蛋白水平在伤后1周明显升高；免疫组化染色后见，间脑核区和视顶盖出现Pax6阳性神经发生巢，以及大量神经前体细胞主要来源Pax6阳性放射状胶质细胞。上述结果说明，单侧眼损伤可引起视神经中枢神经发生反应，神经发生巢的产生可能是虹鳟视神经损伤后修复过程中的代偿现象。

orcid: 0000-0003-0388-3147(Evgeniya V. Pushchina)

关键词: 视神经损伤, neurolin, Zn8轴突再生因子, 转录因子Pax6, 虹鳟, 放射状胶质, 神经再生微环境, 神经再生

Abstract:

In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and actively participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-labeled protein, is involved in the repair of damaged optic nerve in goldfish. At 1 week after unilateral eye injury, the expression of neurolin in the optic nerve and chiasm, and the expression of Pax6 that influences nervous system development in various brain regions in the rainbow trout (Oncorhynchus mykiss) were detected. Immunohistochemical staining revealed that the number of Zn8+ cells in the optic nerve head and intraorbital segment was obviously increased, and the increase in Zn8+ cells was also observed in the proximal and distal parts of injured optic nerve. This suggests that Zn8+ astrocytes participate in optic nerve regeneration. ELISA results revealed that Pax6 protein increased obviously at 1 week post-injury. Immunohistochemical staining revealed the appearance of Pax6+ neurogenic niches and a larger number of neural precursor cells, which are mainly from Pax6+ radial glia cells, in the nuclei of the diencephalon and optic tectum of rainbow trout (Oncorhynchus mykiss). Taken together, unilateral eye injury can cause optic nerve reaction, and the formation of neurogenic niches is likely a compensation phenomenon during the repair process of optic nerve injury in rainbow trout (Oncorhynchus mykiss).

Key words: optic nerve injury, neurolin, Zn8, Pax6, rainbow trout (Oncorhynchus mykiss), radial glia cells, microenvironment, neural regeneration