中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (6): 1364-1368.doi: 10.4103/1673-5374.327358

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

母体表达基因3调节早产儿视网膜病变后的血管新生

  

  • 出版日期:2022-06-15 发布日期:2021-12-17

Maternally expressed gene 3 regulates retinal neovascularization in retinopathy of prematurity

Yu Di, Yue Wang, Yue-Xia Wang, Xue Wang, Yuan Ma, Qing-Zhu Nie*   

  1. Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, China
  • Online:2022-06-15 Published:2021-12-17
  • Contact: Qing-Zhu Nie, PhD, 390532915@qq.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81600747 (to YD), a grant from Liaoning Department of Education, No. QNZR2020010 (to YD) and a grant from 345 Talent Project of Shengjing Hospital (to YD). 

摘要:

已有研究证实,母体表达基因3(MEG3)对糖尿病视网膜病变的保护作用,但其对早产儿视网膜病变后血管新生的作用尚不确定。实验由此拟观察母体表达基因3过表达对氧诱导早产儿视网膜病变小鼠模型视网膜血管新生的作用。结果显示,母体表达基因3过表达可明显抑制早产儿视网膜病变后视网膜血管新生,该作用可能是通过下调PI3K,Akt和血管内皮生长因子及促炎症因子的表达实现的,因而以母体表达基因3为靶点的干预有望成为未来临床治疗早产儿视网膜病变的一种新方法。

https://orcid.org/0000-0003-4749-2084 (Qing-Zhu Nie)

Abstract: The mouse model of oxygen induced retinopathy is suitable for the study of various retinal neovascularization diseases, including retinopathy of prematurity. The maternally expressed gene 3 (MEG3) has been demonstrated to have an inhibitory effect on diabetic retinopathy. In this study, we investigated the role of MEG3 overexpression in oxygen-induced retinopathy in mice. The results showed that MEG3 overexpression effectively inhibited the production of retinal neovascularization in oxygen-induced retinopathy mice. It acts by down-regulating the expression of phosphoinositide 3-kinase, serine/threonine kinase, and vascular endothelial growth factor and pro-inflammatory factors. MEG3 overexpression lentivirus has a future as a new method for the clinical treatment of retinopathy of prematurity. The animal experiments were approved by the Animal Ethics Committee of Shengjing Hospital of China Medical University, China (approval No. 2016PS074K) on February 25, 2016.

Key words: long noncoding RNA, maternally expressed gene 3, neurodevelopment, oxygen-induced retinopathy, phosphoinositide 3-kinase, retinal neovascularization, retinopathy of prematurity, serine/threonine kinase, vascular endothelial growth factor

中图分类号: