中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (8): 1685-1694.doi: 10.4103/1673-5374.332131

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

神经血管单元模型的构建及影像学观察

  

  • 出版日期:2022-08-15 发布日期:2022-01-21
  • 基金资助:
    国家自然科学基金项目(82104412, 81873023);陕西省自然科学基础研究项目(2020JQ-865);陕西省教育厅项目(20JK0597);陕西中医药大学学科创新团队项目(2019-QN02)

Construction and imaging of a neurovascular unit model

Taiwei Dong1, *, Min Li1, Feng Gao1, Peifeng Wei1, Jian Wang2, *   

  1. 1College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi Province, China; 2College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Provinve, China
  • Online:2022-08-15 Published:2022-01-21
  • Contact: Taiwei Dong, PhD, 1484904430@qq.com; Jian Wang, MD, zhongyaoxue2018@163.com.
  • Supported by:
    This work was financially supported by the National Natural Science Foundation of China, Nos. 82104412 (to TD), 81873023 (to JW); Natural Science Basic Research Program of Shaanxi Province of China, No. 2020JQ-865 (to TD); Education Department of Shaanxi Province of China, No. 20JK0597 (to TD); and the Subject Innovation Team of Shaanxi University of Chinese Medicine of China, No. 2019-QN02 (to PW).

摘要:

2001年,卒中进展审查小组会议引入了神经血管单元的概念,强调了其对中枢神经系统血管和神经的健康和疾病状态的重要性。从那时起,神经血管单元得到重视,其对脑卒中和神经退行性疾病的预防、治疗和预后具有重要意义。那么,如何建立起高效、低成本、低能耗的体外神经血管单元模型以及对体内外神经血管单元进行无创观察是目前研究者们亟须解决的重要问题。此次综述首先总结了神经血管单元的构成,然后探索了不同类型干细胞以及细胞共培养方法在构建神经血管单元模型中的效果和差异,最后分析了近年来应用于观察神经血管单元的影像学方法的进展以及在多种中枢神经系统疾病的监测和机制研究中的积极作用。

https://orcid.org/0000-0002-5173-267X (Taiwei Dong)

关键词:

神经血管单元, 血脑屏障, 神经细胞共培养, 脑病研究, 成像技术, 干细胞, 3D打印, 微流控芯片, 计算生物学, 系统评价

Abstract: In 2001, the concept of the neurovascular unit was introduced at the Stroke Progress Review Group meeting. The neurovascular unit is an important element of the health and disease status of blood vessels and nerves in the central nervous system. Since then, the neurovascular unit has attracted increasing interest from research teams, who have contributed greatly to the prevention, treatment, and prognosis of stroke and neurodegenerative diseases. However, additional research is needed to establish an efficient, low-cost, and low-energy in vitro model of the neurovascular unit, as well as enable noninvasive observation of neurovascular units in vivo and in vitro. In this review, we first summarize the composition of neurovascular units, then investigate the efficacy of different types of stem cells and cell culture methods in the construction of neurovascular unit models, and finally assess the progress of imaging methods used to observe neurovascular units in recent years and their positive role in the monitoring and investigation of the mechanisms of a variety of central nervous system diseases.

Key words: 3D printing, blood-brain barrier, computational biology, encephalopathy, imaging techniques, microfluidic on-chip methods, nerve cell co-culture, neurovascular unit, review, stem cells