中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (2): 344-349.doi: 10.4103/1673-5374.346459

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

一种新型双侧纹状体复发性脑出血的老年小鼠模型

  

  • 出版日期:2023-02-15 发布日期:2022-08-08
  • 基金资助:
    广东省自然科学基金项目(2018A030313427),广州市科学技术计划项目(202002030393)

A novel aged mouse model of recurrent intracerebral hemorrhage in the bilateral striatum

Li-Min Wang1, 2, Zhi-Hua Liu3, Hong-Lei Ren4, Xue-Mei Chen5, Jun-Min Wang5, Hui-Min Cai2, Li-Ping Wei2, Hui-Hong Tian2, #br# Jian Wang5, *, Li-Juan Wang1, 2, *#br#   

  1. 1The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong Province, China; 2Department of Neurology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Neuroscience Institute, Guangzhou, Guangdong Province, China; 3School of Medicine, South China University of Technology, Guangzhou, Guangdong Province, China; 4Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China; 5Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China
  • Online:2023-02-15 Published:2022-08-08
  • Contact: Jian Wang, PhD, jianwang2020@outlook.com; Li-Juan Wang, PhD, wljgd68@163.com.
  • Supported by:
    This work was supported by the Natural Science Foundation of Guangdong Province of China, No. 2018A030313427 and the Science and Technology Program of Guangzhou of China, No. 202002030393 (both to LMW).

摘要:

目前的脑卒中的动物模型主要针对单次脑出血,尚缺乏可靠的复发性脑出血模型。实验首先在16月龄老年C57BL/6雄性小鼠左侧纹状体注射胶原酶VII-S建立脑出血模型,21d后在右侧纹状体也注射胶原酶VII-S以模拟复发性脑出血。结果显示,双侧纹状体重复出血小鼠模型在出血早期的神经功能较单侧纹状体出血模型更差,且在后续神经功能逐渐恢复的过程中,运动灵活性,运动协调能力及运动速度的恢复显著延迟,并出现更明显的情绪及认知功能的障碍。提示这一模型可很好地模拟临床上常见的复发性脑出血的特征,或可成为研究复发性脑出血病理过程及治疗靶点的重要工具。

https://orcid.org/0000-0001-6584-8617 (Li-Min Wang); https://orcid.org/0000-0003-2291-640X (Jian Wang)

关键词: 动物模型, 认知障碍, 抑郁样行为, 多巴胺能神经元, 情绪, 脑出血, 运动, 神经功能, 复发性脑出血, 行为学研究

Abstract: The current animal models of stroke primarily model a single intracerebral hemorrhage (ICH) attack, and there is a lack of a reliable model of recurrent ICH. In this study, we established 16-month-old C57BL/6 male mouse models of ICH by injecting collagenase VII-S into the left striatum. Twenty-one days later, we injected collagenase VII-S into the right striatum to simulate recurrent ICH. Our results showed that mice subjected to bilateral striatal hemorrhage had poorer neurological function at the early stage of hemorrhage, delayed recovery in locomotor function, motor coordination, and movement speed, and more obvious emotional and cognitive dysfunction than mice subjected to unilateral striatal hemorrhage. These findings indicate that mouse models of bilateral striatal hemorrhage can well simulate clinically common recurrent ICH. These models should be used as a novel tool for investigating the pathogenesis and treatment targets of recurrent ICH.

Key words: animal model, cognition impairment, depression-like behavior, dopaminergic neurons, emotion, intracerebral hemorrhage, motor, neurologic function, recurrent intracerebral hemorrhage