中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (2): 451-455.doi: 10.4103/1673-5374.346462

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

miR-125a-5p促进调节性T细胞分化对视神经炎的抗炎效应

  

  • 出版日期:2023-02-15 发布日期:2022-08-09
  • 基金资助:
    国家自然科学基金项目(81560162);广西壮族自治区自然科学基金项目(2018GXNSFAA050052)

Anti-inflammatory effect of miR-125a-5p on experimental optic neuritis by promoting the differentiation of Treg cells

Jia-Lin Zhan1, Yan-Ling Huang1, Qiao-Wen Liang1, Xiao-Sheng Qu2, Zi-Mei Dong3, Yi Du1, *, Wen-Jing Luo4, *   

  1. 1Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 2National Engineering Laboratory of Southwest Endangered Medicinal Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi Zhuang Autonomous Region, China; 3Department of Neurology, the People’s Hospital of Chuxiong Yi Autonomous Prefecture, Chuxiong Yi Autonomous Prefecture, Yunnan Province, China; 4Department of Neurology, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China
  • Online:2023-02-15 Published:2022-08-09
  • Contact: Wen-Jing Luo, MD, wenjingluo125@qq.com; Yi Du, MD, duyi@gxmu.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81560162; and the Guangxi Natural Science Foundation of China, No. 2018GXNSFAA050052 (both YD).

摘要:

目前临床上主要采用甲基强的松龙冲击方法治疗视神经炎,其可加速视力恢复,但不能改善长期视力预后。最近有研究发现,miR-125a-5p对自身免疫性疾病具有免疫调节作用,但其对视神经炎是否也发挥作用尚不可知。实验以腺相关病毒转染过表达或沉默小鼠的miR-125a-5p,发现沉默miR-125a-5p会延长视觉诱发电位的潜伏期以及加重视神经的炎症;而miR-125a-5p过表达则可抑制视神经的炎症,保护视网膜神经节细胞并增加调节性T细胞的比例。表明miR-125a-5p可通过促进调节性T细胞的分化而发挥抗炎作用。

https://orcid.org/0000-0002-0632-0182 (Wen-Jing Luo); https://orcid.org/0000-0003-4879-5159 (Yi Du)

关键词: 视神经炎, 微小RNA, 调节性T细胞, 炎症, 水通道蛋白4, 视野缺损, 神经保护, 少突胶质细胞, 皮质类固醇, Th17细胞

Abstract: Methylprednisolone pulse treatment is currently used for optic neuritis. It can speed visual recovery, but does not improve the ultimate visual outcomes. Recent studies have reported that miR-125a-5p has immunomodulatory effects on autoimmune diseases. However, it remains unclear whether miR-125a-5p has effects on optic neuritis. In this study, we used adeno-associated virus to overexpress or silence miR-125a-5p in mice. We found that silencing miR-125a-5p increased the latency of visual evoked potential and aggravated inflammation of the optic nerve. Overexpression of miR-125a-5p suppressed inflammation of the optic nerve, protected retinal ganglion cells, and increased the percentage of Treg cells. Our findings show that miR-125a-5p exhibits anti-inflammatory effects through promoting the differentiation of Treg cells.

Key words: aquaporin-4, corticosteroids, inflammation, microRNA, neuroprotection, oligodendrocyte, optic neuropathy, regulatory T cells, Th17 cell, visual field defect