关键词: font-family:宋体, ">脑缺血再灌注损伤, 原肌球蛋白font-family:Calibri, sans-serif, ">4font-family:宋体, ">, 分子探针, 纳米壳, 光声成像, 诊断, 动态监测, 缺血性脑卒中, 超声, 大脑中动脉闭塞

Abstract: In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge. We injected porous Ag/Au@SiO2 bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography. At each measured time point, the total photoacoustic signal was significantly higher on the affected side than on the healthy side. Twelve hours after reperfusion, cerebral perfusion on the affected side increased, cerebrovascular injury worsened, and anti-tropomyosin 4 expression increased. Twenty-four hours after reperfusion and later, perfusion on the affected side declined slowly and stabilized after 1 week; brain injury was also alleviated. Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes. The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression. 

Key words: cerebral ischemia/reperfusion injury, diagnosis, dynamic monitoring, ischemic stroke, middle cerebral artery occlusion, molecular probe, nanoshells, photoacoustic imaging, tropomyosin 4, ultrasound