中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (3): 657-663.doi: 10.4103/1673-5374.350212

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

透明质酸颗粒水凝胶神经导管可促进大鼠损伤坐骨神经的再生和运动功能的恢复

  

  • 出版日期:2023-03-15 发布日期:2022-08-28
  • 基金资助:
    中国国家自然科学基金项目(81620108008, 31971112,82071373),陕西省创新能力支撑计划项目(2021TD-57)

A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats

Jie Yang1, #, Chia-Chen Hsu2, #, Ting-Ting Cao1, Hua Ye2, 3, *, Jing Chen4, *, Yun-Qing Li1, 4, 5, 6, *   

  1. 1Institute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi Province, China; 2Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK; 3The Oxford Suzhou Centre for Advanced Research (OSCAR), Suzhou, Jiangsu Province, China; 4Department of Anatomy, Histology and Embryology & K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi’an, Shaanxi Province, China; 5Key Laboratory of Brain Science Research and Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, Hainan Province, China; 6Department of Anatomy, College of Basic Medical Sciences, Dali University, Dali, Yunnan Province, China
  • Online:2023-03-15 Published:2022-08-28
  • Contact: Yun-Qing Li, PhD, deptanat@fmmu.edu.cn; Jing Chen, PhD, chenjing@fmmu.edu.cn; Hua Ye, PhD, hua.ye@eng.ox.ac.uk.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, Nos. 81620108008 (to YQL), 31971112 (to YQL), 82071373 (to JC) and Innovation Capability Support Program of Shaanxi, No. 2021TD-57 (to YQL).

摘要:

体外实验发现透明质酸颗粒水凝胶可促进神经元和星形胶质细胞建立细胞集落以及轴突延伸,表明其可模拟细胞外基质结构以促进神经再生;但是目前尚无相应的体内实验的结果。此次实验将含有透明质酸颗粒水凝胶的神经导管移植到大鼠坐骨神经的10mm横断损伤部位,通过BBB运动评分、坐骨神经复合肌肉动作电位记录、荧光金逆行示踪、生长相关蛋白43/S100免疫荧光染色、透射电镜以及腓肠肌湿重比和Masson染色评估结果显示,含有透明质酸颗粒水凝胶的神经导管移植促进损伤坐骨神经轴突和髓鞘再生、电生理功能以及大鼠运动功能恢复的效果与自体神经移植接近,且明显优于透明质酸大块水凝胶和硅胶管。表明含有透明质酸颗粒水凝胶的组织工程导管可有效地促进坐骨神经损伤后的神经形态及功能的恢复,有开发为修复缺损周围神经理想材料的潜力。

https://orcid.org/0000-0002-3707-3348 (Yun-Qing Li); https://orcid.org/0000-0001-9971-1053 (Jing Chen); https://orcid.org/0000-0001-7613-6041 (Hua Ye)

关键词:

神经再生, 周围神经再生, 功能恢复, 透明质酸, 颗粒水凝胶, 坐骨神经损伤, 神经导管, 组织工程, 横断损伤, 髓鞘

Abstract: A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro, suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration. However, in vivo experiments have not been conducted. In this study, we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap. The Basso, Beattie, and Bresnahan locomotor rating scale, sciatic nerve compound muscle action potential recording, Fluoro-Gold retrograde tracing, growth related protein 43/S100 immunofluorescence staining, transmission electron microscopy, gastrocnemius muscle dry/wet weight ratio, and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath, and recovery of the electrophysiological function and motor function as autologous nerve transplantation. The conduit results were superior to those of a bulk hydrogel or silicone tube transplant. These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve. The nerve conduits have the potential as a material for repairing peripheral nerve defects. 

Key words: functional recovery, granular hydrogel, hyaluronic acid, myelin sheath, nerve conduit, nerve regeneration, peripheral nerve regeneration, sciatic nerve injury, tissue engineering, transection injury