中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (7): 1548-1552.doi: 10.4103/1673-5374.385838

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

重复爆炸诱发大鼠创伤性脑损伤后腰段脊髓肾上腺素水平降低

  

  • 出版日期:2024-07-15 发布日期:2023-11-28
  • 基金资助:
    这项工作得到了美国退伍军人事务部康复研究与发展处(RR&D)[B3123-I/ I01 RX003123 和 B3986-R/ I01 RX003986-01A1]的支持

Reduction of epinephrine in the lumbar spinal cord following repetitive blast-induced traumatic brain injury in rats

Shigeharu Tsuda1, 2, Mustafa Golam1, 2, Jiamei Hou1, 2, Kevin K.W. Wang3, Floyd J. Thompson1, 4, Prodip Bose1, 2, 5, *   

  1. 1Brain Rehabilitation Research Center, Malcom Randall VA Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL, USA; 2Department of Anesthesiology, College of Medicine, University of Florida, Gainesville, FL, USA; 3Department of Emergency Medicine, University of Florida, Gainesville, FL, USA; 4Department of Neuroscience, University of Florida, Gainesville, FL, USA; 5Department of Neurology, University of Florida, Gainesville, FL, USA
  • Online:2024-07-15 Published:2023-11-28
  • Contact: Prodip Bose, MD, PhD, pbose@anest.ufl.edu or prodip.bose@va.gov.
  • Supported by:
    This work was supported by the United States Department of Veterans Affairs Rehabilitation Research and Development Service (RR&D) [Merit Review Award numbers B3123-I/ I01 RX003123 and B3986-R/ I01 RX003986-01A1].

摘要:

创伤性脑损伤预后不良与单胺类物质(尤其是肾上腺素)水平失调有关,但很少有临床前研究对创伤性脑损伤后中枢神经系统中的肾上腺素水平进行检测。事实上,多项研究表明,肾上腺素在脑血流的自动调节、适应、增强长期记忆、情感记忆编码和正常肌肉功能方面发挥着关键作用。因此,实验旨在确定轻度重复性爆炸诱导的创伤性脑损伤对大鼠几个特定功能区肾上腺素水平的影响。在3次间隔3d的重复爆炸损伤后,在伤后第8天应用高灵敏度电化学检测器和高效液相色谱法检测了大鼠运动皮质、海马、蓝斑、前庭神经核和腰段脊髓肾上腺素水平。结果显示,与年龄和性别匹配的假手术组相比,创伤性脑损伤大鼠腰段脊髓中肾上腺素水平明显下降;而在其他特定脑区,尽管肾上腺素水平在创伤性脑损伤后略有变化,但两组间比较差异无显著性意义。这是首次报道重复性轻度爆炸诱导的创伤性脑损伤后脊髓中肾上腺素水平的改变;研究成果有助于更好地理解创伤性脑损伤的病理生理学改变,从而开发有针对性的新疗法。

https://orcid.org/0000-0000-1378-3644 (Prodip Bose)

Abstract: Traumatic brain injury-induced unfavorable outcomes in human patients have independently been associated with dysregulated levels of monoamines, especially epinephrine, although few preclinical studies have examined the epinephrine level in the central nervous system after traumatic brain injury. Epinephrine has been shown to regulate the activities of spinal motoneurons as well as increase the heart rate, blood pressure, and blood flow to the hindlimb muscles. Therefore, the purpose of the present study was to determine the impact of repeated blast-induced traumatic brain injury on the epinephrine levels in several function-specific central nervous system regions in rats. Following three repeated blast injuries at 3-day intervals, the hippocampus, motor cortex, locus coeruleus, vestibular nuclei, and lumbar spinal cord were harvested at post-injury day eight and processed for epinephrine assays using a high-sensitive electrochemical detector coupled with high-performance liquid chromatography. Our results showed that the epinephrine levels were significantly decreased in the lumbar spinal cord tissues of blast-induced traumatic brain injury animals compared to the levels detected in age- and sex-matched sham controls. In other function-specific central nervous system regions, although the epinephrine levels were slightly altered following blast-induced traumatic brain injury, they were not statistically significant. These results suggest that blast injury-induced significant downregulation of epinephrine in the lumbar spinal cord could negatively impact the motor and cardiovascular function. This is the first report to show altered epinephrine levels in the spinal cord following repetitive mild blast-induced traumatic brain injury.

Key words: balance, blood flow, cardiovascular system, central nervous system, epinephrine, ischemic damage, lumbar spinal cord, muscle tone, repeated blast, spasticity, traumatic brain injury