中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (12): 2626-2636.doi: 10.4103/NRR.NRR-D-23-01789

• 综述:退行性病与再生 • 上一篇    下一篇

年龄相关性黄斑变性布鲁赫膜模型的生物材料工程策略

  

  • 出版日期:2024-12-15 发布日期:2024-03-30

Biomaterial engineering strategies for modeling the Bruch’s membrane in age-related macular degeneration

Blanca Molins1, *, Andrea Rodríguez1, Víctor Llorenç1, 2, Alfredo Adán1, 2   

  1. 1Group of Ocular Inflammation: Clinical and Experimental Studies, Institut d’Investigacions Biomèdiques Agustí Pi I Sunyer (IDIBAPS), Barcelona, Spain; 2Institut Clínic d’Oftalmologia (ICOF), Hospital Clínic Barcelona, Spain
  • Online:2024-12-15 Published:2024-03-30
  • Contact: Blanca Molins, PhD, bmolins@recerca.clinic.cat.
  • Supported by:
    This work was supported by the Ministry of Science and Innovation of Spain, “Instituto de Salud Carlos III”, “Fondo de Investigación Sanitaria” (PI19/00265), and funds FEDER “Una manera de hacer Europa” (to BM).

摘要: https://orcid.org/0000-0003-0941-9134 (Blanca Molins)

Abstract: Age-related macular degeneration, a multifactorial inflammatory degenerative retinal disease, ranks as the leading cause of blindness in the elderly. Strikingly, there is a scarcity of curative therapies, especially for the atrophic advanced form of age-related macular degeneration, likely due to the lack of models able to fully recapitulate the native structure of the outer blood retinal barrier, the prime target tissue of age-related macular degeneration. Standard in vitro systems rely on 2D monocultures unable to adequately reproduce the structure and function of the outer blood retinal barrier, integrated by the dynamic interaction of the retinal pigment epithelium, the Bruch’s membrane, and the underlying choriocapillaris. The Bruch’s membrane provides structural and mechanical support and regulates the molecular trafficking in the outer blood retinal barrier, and therefore adequate Bruch’s membrane-mimics are key for the development of physiologically relevant models of the outer blood retinal barrier. In the last years, advances in the field of biomaterial engineering have provided novel approaches to mimic the Bruch’s membrane from a variety of materials. This review provides a discussion of the integrated properties and function of outer blood retinal barrier components in healthy and age-related macular degeneration status to understand the requirements to adequately fabricate Bruch’s membrane biomimetic systems. Then, we discuss novel materials and techniques to fabricate Bruch’s membrane-like scaffolds for age-related macular degeneration in vitro modeling, discussing their advantages and challenges with a special focus on the potential of Bruch’s membrane-like mimics based on decellularized tissue.

Key words: age-related macular degeneration, Bruch’s membrane, decellularization, retinal pigment epithelium, scaffold