中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (1): 18-23.

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

Dark rearing maintains tyrosine hydroxylase expression in retinal amacrine cells following optic nerve transection

  

  • 收稿日期:2011-09-20 修回日期:2011-11-25 出版日期:2012-01-05 发布日期:2012-01-05

Dark rearing maintains tyrosine hydroxylase expression in retinal amacrine cells following optic nerve transection

Wei Wan1, 2, Zhenghai Liu2, Xiaosheng Wang1, Xuegang Luo1   

  1. 1 Department of Human Anatomy and Neurobiology, Xiangya Medical College of Central South University, Changsha 410013, Hunan Province, China
    2 Department of Human Anatomy, University of South China, Hengyang 421001, Hunan Province, China
  • Received:2011-09-20 Revised:2011-11-25 Online:2012-01-05 Published:2012-01-05
  • Contact: Xu-egang Luo, Master, Profes-sor, Department of Human Anatomy and Neurobiology, Xiangya Medical College of Central South University, Changsha 410013, Hunan Province, China xgluo@xysm.net
  • About author:Wei Wan☆, Studying for doctorate, Associate profes-sor, Department of Human Anatomy and Neurobiology, Xiangya Medical College of Central South University, Changsha 410013, Hunan Province, China; Department of Human Anatomy, Univer-sity of South China, Hen-gyang 421001, Hunan Prov-ince, China

PDF

815

Abstract:

The present study examined changes in retinal tyrosine hydroxylase (TH) expression in rats having undergone optic nerve transection and housed under a normal day/night cycle or in the dark. The aim was to investigate the effects of amacrine cells on axonal regeneration in retinal ganglion cells and on the synapses that transmit visual signals. The results revealed that retinal TH expression gradually decreased following optic nerve transection in rats housed under a normal day/night cycle, reaching a minimum at 5 days. In contrast, retinal TH expression decreased to a minimum at 1 day following optic nerve transection in dark reared rats, gradually increasing afterward and reaching a normal level at 5-7 days. The number of TH-positive synaptic particles correlated with the TH levels, indicating that dark rearing can help maintain TH expression during the synaptic degeneration stage (5-7 days after optic nerve injury) in retinal amacrine cells.

Key words: optic nerve transection, tyrosine hydroxylase, dark rearing, amacrine cells, peripheral nerve injury, neural regeneration