中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (1): 61-65.

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

Chondroitinase ABC improves recovery of long sciatic nerve defects

  

  • 收稿日期:2011-09-15 修回日期:2011-12-24 出版日期:2012-01-05 发布日期:2012-01-05

Chondroitinase ABC improves recovery of long sciatic nerve defects

Hailong Yu1, Liangbi Xiang1, Wenjing Xu2, Bin Zhao2, Yu Wang2, Jiang Peng2, Shibi Lu2   

  1. 1Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China
    2 Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2011-09-15 Revised:2011-12-24 Online:2012-01-05 Published:2012-01-05
  • Contact: Jiang Peng, Doctor, Associate researcher, Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China pengjdxx@126.com
  • About author:Hailong Yu☆, Doctor, Attending physician, Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China Hailong Yu and Liangbi Xiang contributed equally to this article.

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Abstract:

Sciatic nerves from allogeneic Sprague-Dawley rats were pretreated with chondroitinase ABC and were used to bridge damaged sciatic nerves in Wistar rats. Chondroitin sulfate proteoglycans were removed from the chemically extracted acellular nerves. At 3 months after grafting, the footplate pinch test result was positive in the Wistar rats. Autotomy scores decreased, and increased muscular contraction tension appeared when triceps surae muscles were stimulated. In addition, the recovery rate of wet triceps surae muscle weight increased, and the distal segment of the chondroitinase ABC-treated graft exhibited Schwann cells next to the nerve fibers. These results suggested that chondroitinase ABC pretreatment enhanced repair of long nerve defects via acellular nerve grafting.

Key words: chondroitinase ABC, neural regeneration, peripheral nerve, sciatic nerve, Schwann cell