中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (4): 278-282.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Increased CD133+ cell infiltration in the rat brain following fluid percussion injury

  

  • 收稿日期:2011-09-24 修回日期:2011-12-22 出版日期:2012-02-05 发布日期:2012-02-05

Increased CD133+ cell infiltration in the rat brain following fluid percussion injury

Ming Wei1, 2, Ziwei Zhou1, Shenghui Li1, Chengwei Jing2, Dashi Zhi1, Jianning Zhang1   

  1. 1 Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China
    2 Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2011-09-24 Revised:2011-12-22 Online:2012-02-05 Published:2012-02-05
  • Contact: Jianning Zhang, M.D., Chief physician, Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China jianningzhang@hotmail.com
  • About author:Ming Wei☆, M.D., Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China; Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China Ming Wei, Shenghui Li and Ziwei Zhou contributed equally to this work.

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Abstract:

The prominin-1/CD133 epitope is expressed in undifferentiated cells. Studies have reported that craniocerebral trauma in animal models of fluid percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133+ cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133+ cells were mainly distributed in hippocampal CA1-3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.

Key words: prominin-1, immunohistochemistry, reverse transcription-PCR, traumatic brain injury, neural regeneration