1 Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China
2 School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
Contact:
Bing Zhu, M.D., Ph.D., Professor,Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700,China; Peijing Rong, M.D.,Ph.D., Professor, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700,China zhubing@mail.cintcm.ac.cn; rongpj@mail.cintcm.ac.cn
About author:Xiaoxuan Ren☆, M.D.,Ph.D., Senior experimentalist, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700,China; School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China Shaojun Wang and Xiaoxuan Ren contributed equally to this study.
1.Spontaneous discharges from individual neurons encountered within the medial preoptic area were recorded extracellularly using glass microelectrodes.
2.Antidromic stimulation was performed using a side-by-side bipolar stimulating electrode placed into the median eminence. Individual neurons at the recording site in the medial preoptic area in female rats were found to be the subpopulation of gonadotropin-releasing hormone neurons.
3.Spontaneous discharges were significantly increased in female rats during proestrus/estrus after sexual stimuli, and displayed characteristics of exogenous hormone feedback inhibition.
1.实验应用玻璃微电极于细胞外记录动情期雌鼠下丘脑视前区内单个神经元的自发性放电特征。
2.通过将并列式双极电极逆向刺激雌鼠生殖器,所记录到的雌鼠下丘脑视前区内单个神经元即为促性腺激素释放激素神经元亚群。
3.动情前期/动情期雌鼠在性刺激后自发放电明显增多,具有外源性激素反馈抑制特性。