中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (30): 2355-2361.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

茶多酚对脑缺血再灌注损伤海马2种DNA修复蛋白表达的影响

  

  • 收稿日期:2012-04-20 修回日期:2012-08-22 出版日期:2012-10-25 发布日期:2012-10-25

Tea polyphenols increase X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 expression in the hippocampus of rats during cerebral ischemia/reperfusion injury

Zhi Wang 1, 2, Rongliang Xue1, Xi Lei1, Jianrui Lv1, Gang Wu1, Wei Li1, Li Xue1, Xiaoming Lei1, Hongxia Zhao1, Hui Gao1, Xin Wei1   

  1. 1 Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China 2 Department of Anesthesiology, Stomatological Hospital, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2012-04-20 Revised:2012-08-22 Online:2012-10-25 Published:2012-10-25
  • Contact: Ron-gliang Xue, Professor, De-partment of Anesthesiology, Second Affiliated Hospital of Medical College, Xi’an Jiao-tong University, Xi’an 710004, Shaanxi Province, China wz9602@sina.com
  • About author:Zhi Wang★, Master, Attend-ing physician, Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China; Department of Anesthesiolo-gy, Stomatological Hospital, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China

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Abstract:

Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.

Key words: global cerebral ischemia/reperfusion, X-ray repair cross-complementing protein 1, apurinic/apyrimidinic endonuclease/redox factor-1, tea polyphenols