中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 266-270.doi: 10.4103/1673-5374.152381

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

环孢霉素A早期干预可延缓周围神经注射损伤后的轴突退变

  

  • 收稿日期:2014-11-22 出版日期:2015-02-17 发布日期:2015-02-17

Early cyclosporin A treatment retards axonal degeneration in an experimental peripheral nerve injection injury model

Ibrahim Erkutlu 1, Mehmet Alptekin 1, Sirma Geyik 2, Abidin Murat Geyik 3, Inan Gezgin 4, Abdulvahap Gök 1    

  1. 1 Department of Neurosurgery, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey
    2 Department of Neurology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey
    3 Department of Neurosurgery, Sanko University, Gaziantep, Turkey
    4 Department of Neurosurgery, Private Park Hospital, Ad?yaman, Turkey
  • Received:2014-11-22 Online:2015-02-17 Published:2015-02-17
  • Contact: Sirma Geyik, M.D., drsirmageyik@hotmail.com.

摘要:

已有研究证实,免疫抑制剂环孢霉素A对周围神经挤压伤后瓦勒变性有延缓作用,但其对药物注射性周围神经损伤的作用尚未见报道。实验为评估环孢霉素A对青霉素钾注射致大鼠周围神经损伤影响的时效性。分别于大鼠坐骨神经青霉素钾注射损伤后30min,8和24h腹腔注射环孢霉素A,发现只有损伤后30min腹腔注射环孢霉素A的大鼠,损伤后4周坐骨神经瓦勒变性程度明显减轻,复合肌肉动作电位波幅明显升高。说明环孢霉素A早期干预对青霉素钾注射致周围神经损伤有神经保护作用。

关键词: 神经再生, 周围神经损伤, 注射损伤, 环孢霉素A, 青霉素钾, 瓦勒变性, 神经电生理

Abstract:

Injury to peripheral nerves during injections of therapeutic agents such as penicillin G potassium is common in developing countries. It has been shown that cyclosporin A, a powerful immunosuppressive agent, can retard Wallerian degeneration after peripheral nerve crush injury. However, few studies are reported on the effects of cyclosporin A on peripheral nerve drug injection injury. This study aimed to assess the time-dependent efficacy of cyclosporine-A as an immunosuppressant therapy in an experimental rat nerve injection injury model established by penicillin G potassium injection. The rats were randomly divided into three groups based on the length of time after nerve injury induced by cyclosporine-A administration (30 minutes, 8 or 24 hours). The compound muscle action potentials were recorded pre-injury, early post-injury (within 1 hour) and 4 weeks after injury and compared statistically. Tissue samples were taken from each animal for histological analysis. Compared to the control group, a significant improvement of the compound muscle action potential amplitude value was observed only when cyclosporine-A was administered within 30 minutes of the injection injury (P < 0.05); at 8 or 24 hours after cyclosporine-A administration, compound muscle action potential amplitude was not changed compared with the control group. Thus, early immunosuppressant drug therapy may be a good alternative neuroprotective therapy option in experimental nerve injection injury induced by penicillin G potassium injection.

Key words: nerve regeneration, peripheral nerve injury, injection injury, cyclosporine A, penicillin G potassium, Wallerian degeneration, neuroelectrophysiology, neural regeneration