中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (5): 878-884.doi: 10.4103/1673-5374.295348

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

周围神经损伤后近端与远端细胞因子表达差异的生物信息学分析

  

  • 出版日期:2021-05-15 发布日期:2020-12-29
  • 基金资助:

    国家自然科学基金项目(31771052);国家重点研发计划(2017YFA0104702);军事医学科学青年培养计划(16QNP144

Bioinformatic analysis of cytokine expression in the proximal and distal nerve stumps after peripheral nerve injury

Xiao-Qing Cheng1, 2, 3, #, Wen-Jing Xu1, 2, 3, #, Xiao Ding1, 2, 3, Gong-Hai Han1, 2, 3, Shuai Wei1, 2, 3, Ping Liu1, 2, 3, Hao-Ye Meng1, 2, 3, Ai-Jia Shang4, 5, *, Yu Wang1, 2, 3, 5, *, Ai-Yuan Wang1, 2, 3, *   

  1. 1 Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China;  2 Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Chinese PLA General Hospital, Beijing, China;  3 Key Lab of Musculoskeletal Trauma & War Injuries, Chinese PLA General Hospital, Beijing, China;  4 Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China;  5 Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
  • Online:2021-05-15 Published:2020-12-29
  • Contact: Ai-Jia Shang, shangaj@126.com; Yu Wang, wangwangdian628@126.com; Ai-Yuan Wang, wangaiyuan301@126.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 31771052 (to YW), the National Key R&D Program of China, No. 2017YFA0104702 (to AJS), and the Youth Cultivation Project of Military Medical Science, No. 16QNP144 (to YW).

摘要:

作者既往研究使用微阵列分析了周围神经损伤后远端残端中细胞因子和表征蛋白的动态表达。(1)实验构建了右侧坐骨神经横断大鼠模型,利用蛋白芯片技术分析损伤后1,7,14和28d时细胞因子表达的变化;(2)结果发现神经横断后1-14d,白细胞介素在神经远端残端表达增加,而生长因子和生长因子相关蛋白主要在神经近端残端上调;Metascape分析结果显示,神经远端残端的炎症反应、凋亡反应和细胞粘附的P值高于近端,但血管生成的P值低于近端;神经远端残端中与轴突相关的细胞因子数量多于近端,且与轴突相关的细胞因子比例则低于近端;(3)实验的可视化结果展示了细胞因子在近端和远端残端中的特定表达模式及差异,或可为临床找到治疗周围神经损伤的潜在疗法提供线索。实验于2016年9月7日经解放军总医院动物伦理委员会批准,批准号:2016-x9-07。

https://orcid.org/0000-0002-8578-5040 (Ai-Yuan Wang)

关键词:

近端残端, 远端残端, 周围神经损伤, 细胞因子, 微阵列技术, 微环境, 基因本体论, 京都基因与基因组百科全书

Abstract: In our previous study, we investigated the dynamic expression of cytokines in the distal nerve stumps after peripheral nerve injury using microarray analysis, which can characterize the dynamic expression of proteins. In the present study, we used a rat model of right sciatic nerve transection to examine changes in the expression of cytokines at 1, 7, 14 and 28 days after injury using protein microarray analysis. Interleukins were increased in the distal nerve stumps at 1–14 days post nerve transection. However, growth factors and growth factor-related proteins were mainly upregulated in the proximal nerve stumps. The P-values of the inflammatory response, apoptotic response and cell-cell adhesion in the distal stumps were higher than those in the proximal nerve stumps, but the opposite was observed for angiogenesis. The number of cytokines related to axons in the distal stumps was greater than that in the proximal stumps, while the percentage of cytokines related to axons in the distal stumps was lower than that in the proximal nerve stumps. Visualization of the results revealed the specific expression patterns and differences in cytokines in and between the proximal and distal nerve stumps. Our findings offer potential therapeutic targets and should help advance the development of clinical treatments for peripheral nerve injury. Approval for animal use in this study was obtained from the Animal Ethics Committee of the Chinese PLA General Hospital on September 7, 2016 (approval No. 2016-x9-07).

Key words: cytokine, distal stumps, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes Pathway, microarray, microenvironment, peripheral nerve injury, proximal stumps