中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (4): 664-669.doi: 10.4103/1673-5374.180755

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

Y型神经导管结合人脐带干细胞适合修复混合神经分叉处缺损

  

  • 收稿日期:2015-11-03 出版日期:2016-04-30 发布日期:2016-04-30
  • 基金资助:

    863项目（2012AA020905）；国家自然科学基金项目（81360194）；973项目（2014CB542200）

Combined use of Y-tube conduits with human umbilical cord stem cells for repairing nerve bifurcation defects

Aikeremujiang•Muheremu1, 2, 3, Jun-gang Sun4, Xi-yuan Wang2, Fei Zhang3, Qiang Ao2, *, Jiang Peng5, *   

  1. 1 Medical Center, Tsinghua University, Beijing, China
    2 Department of Tissue Engineering, China Medical University, Shenyang, Liaoning Province, China
    3 Department of Orthopedics, Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China
    4 Department of Orthopedics, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China
    5 Institute of Orthopaedics, General Hospital of PLA, Beijing, China
  • Received:2015-11-03 Online:2016-04-30 Published:2016-04-30
  • Contact: Qiang Ao, M.D., Ph.D. or Jiang Peng, M.D., Ph.D., aoqiang@tsinghua.edu.cn or pengjdxx@126.com.
  • Supported by:

    This study was funded by the National High Technology Research and Development Program of China (“863”Program, No. 2012AA020905), the National Natural Science Foundation of China (No. 81360194), and the National Basic Research Program of China (973 program, No. 2014CB542200).

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摘要：

由于神经干分叉点结构复杂，该部位断端吻合缝合修复后再生的神经轴突很容易进入错误的远端神经残端，导致不良结果，而Y型神经导管可能有效解决上述问题，但哪种Y型神经导管的效果更佳目前尚无结论。为此，实验建立了大鼠股神经分叉处损伤模型，并以含人脐血干细胞的不同类型的硅胶Y型神经导管进行修复。12周后，不同类型的Y型神经导管修复后大鼠股四头肌质量和股神经超微结构接近，但经长分支短主干的Y型神经导管修复后的股神经电生理功能及再生神经出芽方向正确的比例最好。说明不同类型的Y型神经导管结合人脐带干细胞均能有效修复混合周围神经分叉处缺损，以长分支短主干的Y型神经导管更适合修复此处神经损伤。

orcid: 0000-0002-1309-1971 (Qiang Ao)

关键词: 神经再生, 周围神经损伤, 神经导管, 选择性神经再生, 趋化性, 人脐血干细胞, 干细胞移植

Abstract:

Given the anatomic complexity at the bifurcation point of a nerve trunk, enforced suturing between stumps can lead to misdirection of
nerve axons, thereby resulting in adverse consequences. We assumed that Y-tube conduits injected with human umbilical cord stem cells
could be an effective method to solve such problems, but studies focused on the best type of Y-tube conduit remain controversial. Therefore,
the present study evaluated the applicability and efficacy of various types of Y-tube conduits containing human umbilical cord stem
cells for treating rat femoral nerve defects on their bifurcation points. At 12 weeks after the bridging surgery that included treatment with
different types of Y-tube conduits, there were no differences in quadriceps femoris muscle weight or femoral nerve ultrastructure. However,
the Y-tube conduit group with longer branches and a short trunk resulted in a better outcome according to retrograde labeling and electrophysiological
analysis. It can be concluded from the study that repairing a mixed nerve defect at its bifurcation point with Y-tube conduits,
in particular those with long branches and a short trunk, is effective and results in good outcomes.

Key words: nerve regeneration, peripheral nerve injury, nerve conduit, selective nerve regeneration, chemotaxis, human umbilical cord blood stem cell, stem cell transplantation, neural regeneration