中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (7): 1159-1164.doi: 10.4103/1673-5374.187057

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

人参皂苷Rg1可对抗过氧化氢诱导人神经母瘤细胞的损伤

  

  • 出版日期:2016-07-30 发布日期:2016-07-30
  • 基金资助:
    北京科委科技计划研发攻关基金(Z111107067311022)

Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells

Zhi-gao Sun1, Li-ping Chen2, *, Fa-wei Wang2, Cheng-yong Xu1, Miao Geng3   

  1. 1 Department of Traditional Chinese Medicine, Hainan Branch Hospital of Chinese PLA General Hospital, Sanya, Hainan Province, China 2 Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing, China 3 Institute of Gerontology, Chinese PLA General Hospital, Beijing, China
  • Online:2016-07-30 Published:2016-07-30
  • Contact: Li-ping Chen, M.D., lipingschen@aliyun.com.
  • Supported by:
    This research was supported by the Research and Development Project Fund of Science and Technology Plan Program of Science and Technology Bureau of Beijing of China, No. Z111107067311022.

PDF

643

摘要：

研究表明，药用植物人参的活性成分人参皂苷Rg1具有清除积累的自由基和提高机体抗氧化能力的作用，实验以此假设人参皂苷Rg1可对抗氧化氢体外诱导人神经母瘤细胞的损伤。故以不同浓度人参皂苷Rg1(50，100 μM)预处理H2O2(150 μM)诱导损伤的人神经母瘤细胞SH-SY5Y，发现人参皂苷Rg1可提高H2O2诱导损伤SH-SY5Y细胞的存活率，减少乳酸脱氢酶漏出量，增加超氧化物歧化酶的活性，有效抑制caspase-3免疫反应，并促进热休克蛋白70基因的表达，作用呈剂量依赖性。结果证实人参皂苷Rg1有保护H2O2诱导的人神经母瘤细胞SH-SY5Y细胞损伤的作用，其机制与抗氧化和抑制细胞凋亡有关。

关键词: 神经再生, 中医药, 中药单体, 人参皂苷Rg1, 人神经母瘤细胞SH-SY5Y, 过氧化氢, 脑缺血, 细胞凋亡, 乳酸脱氢酶, 超氧化物歧化酶, 凋亡调控因子, 热休克蛋白70, 量效关系

Abstract: The active ingredient of ginseng, ginsenosides Rg1, has been shown to scavenge free radicals and improve antioxidant capacity. This study hypothesized that ginsenosides Rg1 has a protective role in human neuroblastoma cells injured by H2O2. Ginsenosides Rg1 at different concentrations (50 and 100 μM) was used to treat H2O2 (150 μM)-injured SH-SY5Y cells. Results demonstrated that ginsenoside Rg1 elevated the survival rate of SH-SY5Y cells injured by H2O2, diminished the amount of leaked lactate dehydrogenase, and increased superoxide dismutase activity. Ginsenoside Rg1 effectively suppressed caspase-3 immunoreactivity, and contributed to heat shock protein 70 gene expression, in a dose-dependent manner. These results indicate that ginsenoside Rg1 has protective effects on SH-SY5Y cells injured by H2O2 and that its mechanism of action is associated with anti-oxidation and the inhibition of apoptosis.

Key words: nerve regeneration, traditional Chinese medicine monomer, ginsenoside Rg1, SH-SY5Y cells, H2O2, cerebral ischemia, cell apoptosis, lactate dehydrogenase, superoxide dismutase, caspase-3, heat shock protein 70, dose-effect relationship, neural regeneration