中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (2): 350-356.doi: 10.4103/1673-5374.286975

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

山楂叶总黄酮可抑制脊髓损伤后的细胞凋亡并促进运动功能恢复

  

  • 出版日期:2021-02-15 发布日期:2020-12-04
  • 基金资助:
    国家自然科学基金（81860391）；广西壮族自治区自然科学基金（2018GXNSFAA281144）；广西壮族自治区重点研究开发项目（桂科AB18221021

Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury

Qiong Zhang1, #, Yin Xiong1, Bo Li1, #, Gui-Ying Deng3, Wen-Wen Fu1, Bai-Chuan Cao2, Shao-Hui Zong2, 3, *, Gao-Feng Zeng1, *   

  1. 1 School of Public Health Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China;   2 Department of Spine Osteopathic Surgery, The First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, China;   3 Research Center for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China
  • Online:2021-02-15 Published:2020-12-04
  • Contact: Shao-Hui Zong, PhD, 379244544@qq.com; Gao-Feng Zeng, PhD, 1685858372@qq.com. #Both authors contributed equally to this study.
  • Supported by:
    The study was supported by the National Natural Science Foundation of China, No. 81860391 (to GFZ); the Natural Science Foundation of Guangxi Zhuang Autonomous Region of China, No. 2018GXNSFAA281144 (to GFZ); the Key Research and Development Project of Guangxi Zhuang Autonomous Region of China, No. guikeAB18221021 (to SHZ).

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摘要：

已有研究显示，黄酮类物质具有治疗脊髓损伤的潜力，山楂叶总黄酮中的黄酮种类丰富且含量高，但当前利用山楂叶总黄酮治疗脊髓损伤的研究报道较少。为此，实验通过Allen’s打击法构建脊髓损伤大鼠模型，分别通过腹腔注射0.2 mL不同浓度的山楂叶总黄酮(5，10，20 mg/kg)，每隔6 h给药1次，3次/d，连续给药14 d。(1) 病理组织染色和BBB评分检测发现，经各剂量山楂叶总黄酮治疗后，脊髓损伤大鼠损伤脊髓组织的病变腔、凋亡细胞逐渐减少，髓鞘、神经细胞核形态排列趋于规则，神经细胞内的尼氏体均有所增加，BBB评分均升高；(2)免疫印迹检测发现，治疗后大鼠损伤脊髓组织中促细胞凋亡蛋白Bax和活化的caspase-3蛋白表达下调，而抑细胞凋亡蛋白Bcl-2蛋白表达上调，且以上指标的改善程度与山楂叶总黄酮的浓度呈剂量-反应关系。(3)上述数据显示了，山楂叶总黄酮可抑制脊髓损伤后细胞凋亡，发挥神经保护，改善脊髓损伤大鼠运动恢复功能的作用。

https://orcid.org/0000-0001-5972-1820 (Shao-Hui Zong); 

0000-0002-6200-0757 (Gao-Feng Zeng)

关键词: 细胞凋亡, Bax蛋白, Bcl-2蛋白, 活化的caspase-3, 炎症, 运动功能恢复, 神经保护, 尼氏体, 脊髓损伤, 山楂叶总黄酮

Abstract: Flavonoids have been reported to have therapeutic potential for spinal cord injury. Hawthorn leaves have abundant content and species of total flavonoids, and studies of the effects of the total flavonoids of hawthorn leaves on spinal cord injury have not been published in or outside China. Therefore, Sprague-Dawley rats were used to establish a spinal cord injury model by Allen’s method. Rats were intraperitoneally injected with 0.2 mL of different concentrations of total flavonoids of hawthorn leaves (5, 10, and 20 mg/kg) after spinal cord injury. Injections were administered once every 6 hours, three times a day, for 14 days. After treatment with various concentrations of total flavonoids of hawthorn leaves, the Basso, Beattie, and Bresnahan scores and histological staining indicated decreases in the lesion cavity and number of apoptotic cells of the injured spinal cord tissue; the morphological arrangement of the myelin sheath and nerve cells tended to be regular; and the Nissl bodies in neurons increased. The Basso, Beattie, and Bresnahan scores of treated spinal cord injury rats were increased. Western blot assays showed that the expression levels of pro-apoptotic Bax and cleaved caspase-3 were decreased, but the expression level of the anti-apoptotic Bcl-2 protein was increased. The improvement of the above physiological indicators showed a dose-dependent relationship with the concentration of total flavonoids of hawthorn leaves. The above findings confirm that total flavonoids of hawthorn leaves can reduce apoptosis and exert neuroprotective effects to promote the recovery of the motor function of rats with spinal cord injury. This study was approved by the Ethics Committee of the Guangxi Medical University of China (approval No. 201810042) in October 2018. 

Key words: apoptosis, Bax protein, Bcl-2 protein, cleaved caspase-3, inflammation, motor function recovery, neuroprotection, Nissl bodies, spinal cord injury, total flavonoids of hawthorn leaves