中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (9): 4275-4289.doi: 10.4103/NRR.NRR-D-25-00621

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

复合生物活性多层纳米纤维硬脑膜促进创伤性脑损伤的修复

  

  • 出版日期:2026-09-15 发布日期:2026-05-21

Complex bioactive nanofibrous dura mater promotes the repair of traumatic brain injury

Siyu Chen1, Xiaopei Zhang1, 2, Qingxia Guo1, Yuying Yan1, Manfei Fu1, Yuanfei Wang3, *, Tong Wu1, 2, *   

  1. 1The Affiliated Hospital of Qingdao University, School of Basic Medicine, Qingdao Medical College, Qingdao University, Qingdao, Shandong Province, China; 
    2Shandong Key Laboratory of Medical and Health Textile Materials, College of Textile & Clothing, Qingdao University, Qingdao, Shandong Province, China; 
    3Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, Shandong Province, China
  • Online:2026-09-15 Published:2026-05-21
  • Contact: Yuanfei Wang, PhD, zhizunbao19@163.com; Tong Wu, PhD, twu@qdu.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 32171322 (to TW); Special Funds for Taishan Scholars Project of Shandong Province, No. tsqn202211125 (to TW); the Natural Science Foundation of Shandong Province, No. ZR2024JQ026 (to TW); Young Elite Scientists Sponsorship Program by CAST, No. YESS20200097 (to TW); Qingdao Key Health Discipline Development Fund, No. 2025-2027 (to YW); Qingdao Clinical Research Center for Oral Diseases, No. 22-3-7-lczx-7-nsh (to YW); Shandong Provincial Key Medical and Health Discipline of Oral Medicine, No. 2025-2027 (to YW); and the “Advanced Biomaterials and Regenerative Medicine (ABRM)” Innovation Team supported by the Young-Talent Introduction and Cultivation Plan in the Universities of Shandong Province (to TW).

PDF

1

摘要：

创伤性脑损伤手术后的硬脑膜闭合对于保持大脑的结构完整性至关重要，并可作为防止感染和脑脊液泄漏的屏障。虽然现有的人造硬脑膜也可提供屏障作用，但其在继发性损伤阶段对受损神经细胞的修复和神经保护性能并不理想。因此，此次实验通过静电纺丝技术成功制备了含有米诺环素和胰岛素样生长因子1的多层纳米纤维硬脑膜，用以修复创伤性脑损伤。结果显示，这种多层纳米纤维硬脑膜能够促进神经突横断、缺氧和葡萄糖剥夺以及氧化应激损伤后SH-SY5Y细胞的存活和神经突延伸。由于其纤维中掺入了盐酸米诺环素和胰岛素生长因子1因而可实现差异化双重释放，用于早期免疫调节，并在修复过程中发挥神经保护作用。此外，多层纳米纤维硬脑膜还能促进小胶质细胞的M2极化和抗炎细胞因子的分泌以及神经细胞的存活。同时多层纳米纤维硬脑膜的抗菌、抗粘附、阻隔、抗渗漏和生物相容性也得到了验证。因此，这种含有米诺环素和胰岛素样生长因子1的多层纳米纤维硬脑膜显示出作为硬脑膜替代物和促进创伤性脑损伤后神经恢复的巨大潜力。


https://orcid.org/0000-0003-4134-307X (Yuanfei Wang); 

https://orcid.org/0000-0001-8822-1868 (Tong Wu)

关键词: 抗粘连, 抗菌, 抗炎, 防泄漏, 屏障性能, 生物相容性, 差异双重释放, 多层纳米纤维硬脑膜, 神经保护, 创伤性脑损伤, 神经再生

Abstract: Dura closure following surgery for traumatic brain injury is important to maintain the structural integrity of the brain and serve as a barrier to prevent infection and leakage of cerebrospinal fluid. Although an artificial dural mater can provide barrier capabilities, its performance in the repair of injured neural cells and neuroprotection during the secondary injury stage can be improved. Therefore, we designed and manufactured a multi-layer nanofibrous dura mater containing minocycline and insulin-like growth factor 1 using electrospinning technology to repair tissue following traumatic brain injury. The results showed that the multi-layer nanofibrous dura mater promoted neuronal process transection, hypoxia, and glucose deprivation, as well as survival and neurite extension of SH-SY5Y cells after oxidative stress injury. Minocycline hydrochloride and insulin growth factor 1 were separately incorporated into the fibers to facilitate their differential dual release for immunomodulation during the early stage of traumatic brain injury and provide neuroprotection during the repair process. In addition, the multi-layered nanofibrous dura mater promoted the increase in M2 polarization for microglia and the secretion of anti-inflammatory cytokines, which enhanced neural cell survival. Furthermore, we verified the antibacterial, anti-adhesion, barrier performance, anti-leakage, and biocompatibility capabilities of the multi-layered nanofibrous dura mater. Therefore, our multi-layered nanofibrous dura mater containing minocycline and insulin-like growth factor 1 has great potential as a substitute for dura mater and promotes nerve recovery following traumatic brain injury.

Key words: anti-adhesion, antibacterial, anti-inflammatory, anti-leakage, barrier performance, biocompatibility, differential dual-release, multilayered nanofibrous dura mater, neuroprotection, traumatic brain injury, nerve regeneration