中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (12): 2160-2163.doi: 10.4103/1673-5374.241467

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

青春期大鼠海马表观遗传标志受性别和一天中时间的调节

  

  • 收稿日期:2018-08-02 出版日期:2018-12-15 发布日期:2018-12-15
  • 基金资助:

    这项工作得到了来自Conselho Nacional deDesenvolvimentoCientíficoeTecnológico - CNPq / Brazil的476634 / 2013-0号的部分支持； CNPq奖学金; CoordenaçãodeAperfeiçoamentodePessoaldeNívelSuperior - CAPES奖学金（致LCM）; Programa de BolsasdeIniciaçãoCientífica - UFRGS

Epigenetic marks are modulated by gender and time of the day in the hippocampi of adolescent rats: a preliminary study

Viviane Rostirola Elsner1, 2, 3, Laura Reck Cechinel1, Louisiana Carolina Ferreira de Meireles1, Karine Bertoldi1, Ionara Rodrigues Siqueira1   

  1. 1 Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
    2 Programa de Pós-Graduação em Biociências e Reabilitação, Centro Universitário Metodista-IPA, Porto Alegre, Rio Grande do Sul, Brasil
    3 Curso de Fisioterapia, Centro Universitário Metodista-IPA, Porto Alegre, Rio Grande do Sul, Brasil
  • Received:2018-08-02 Online:2018-12-15 Published:2018-12-15
  • Contact: Ionara Rodrigues Siqueira, ionara@ufrgs.br.
  • Supported by:

    This work was partially supported by grant 476634/2013-0 from Conselho Nacional de Desenvolvimento Científico e Tecnológico -CNPq /Brazil. CNPq fellowships (to IRS, VRE, and KB); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES fellowships (to LCM); Programa de Bolsas de Iniciação Científica – UFRGS (to LRC).

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摘要：

新生大鼠性别影响外周组织中的表观遗传特征，但青少年期间大脑区域的性别相关表观遗传特征却少有报道。实验旨在探讨性别和一天中不同时间点（特别是清晨和下午）对青春期大鼠海马DNA甲基转移酶1含量和组蛋白去乙酰化酶（HDAC）活性的影响。结果发现，与雄性大鼠相比，雌性大鼠的海马中的两种表观遗传标志物水平均明显增加，这提示转录活性降低。同时，与下午的雄性和雌性大鼠相比，清晨时大鼠海马组蛋白去乙酰化酶活性更高，而DNA甲基转移酶1的含量在一天中未发生改变。结果表明，在青春期大鼠海马DNA甲基化和组蛋白乙酰化状态可能受性别影响，而一天中的时间只影响组蛋白去乙酰化酶活性。

orcid:0000-0002-4460-0978(Ionara Rodrigues Siqueira)

关键词: 青春期大鼠, DNA甲基转移酶1, 组蛋白去乙酰化酶, 海马, 一天中的时间

Abstract:

Although the involvement of gender in epigenetic machinery in peripheral tissues during the neonatal period has been suggested, the gender-related epigenetic profile of brain areas during the adolescent period is rarely exploited. Furthermore, the influence of time of day on hippocampal acetylation marks has been demonstrated in young adult and aged rats; however, there are no studies reporting epigenetic changes in the adolescent period. Therefore, this study aimed to investigate the effects of gender on hippocampal DNA methyltransferase 1 content and histone deacetylase (HDAC) activity of adolescent rats at different time points, specifically early morning and afternoon. Both epigenetic markers increased significantly in the hippocampi of female rats compared to the male group, an indicator of reduced transcriptional activity. In addition, HDAC activity during the early morning was higher compared to afternoon groups in both male and female rats, while DNA methyltransferase 1 content was not altered by the time of day. Our findings demonstrate that hippocampal DNA methylation and histone acetylation status can be influenced by gender during the adolescent period, while the time of the day impacts HDAC activity.

Key words: adolescent rats, DNA methyltransferase 1, histone deacetylase, hippocampus, time of the day, gender, epigenetic marks