中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (11): 2020-2024.doi: 10.4103/1673-5374.259627

• 原著:视神经损伤修复保护与再生 • 上一篇    

抗氧化是依托咪酯保护视神经切断损伤视网膜神经节细胞的作用途径

  

  • 出版日期:2019-11-15 发布日期:2019-11-15
  • 基金资助:

    中国国家自然科学基金项目（81670846，81470631），陕西省自然科学基金项目（2016SF-171），国家重点基础研究发展计划(973)项目（2014CB542202）

Etomidate affects the anti-oxidant pathway to protect retinal ganglion cells after optic nerve transection

Xuan Zhao 1, 2 , Fang Kuang 1 , Yi-Yan You 3 , Ming-Mei Wu 1 , Si-Wei You 1   

  1. 1 Department of Neurobiology and Collaborative Innovation Center for Brain Science, Air Force Medical University, Xi’an, Shaanxi Province, China
    2 Department of Histology and Embryology & Shaanxi Key Laboratory of Brain Disorders, Xi’an Medical University, Xi’an, Shaanxi Province, China
    3 Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ, USA
  • Online:2019-11-15 Published:2019-11-15
  • Contact: Si-Wei You, PhD, yousiwei@hotmail.com; Ming-Mei Wu, PhD, wumm33@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81670846 (to MMW) and 81470631 (to SWY); the Natural Science Foundation of Shaanxi Province of China, No. 2016SF-171 (to MMW), and the National Basic Research Program of China, No. 2014CB542202 (to SWY).

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摘要：

作者既往研究表明，非巴比妥类静脉麻醉剂依托咪酯对视神经切断后7d内的视网膜神经节细胞有保护作用，但这一过程是否与抗氧化应激反应有关尚不明确。为揭示其作用机制，实验建立了成年雄性大鼠左眼后方1mm处视神经横断损伤模型大鼠。(1) 每天给与视神经损伤模型大鼠腹膜注射依托咪酯（4mg/kg）1次，持续7d；(2) 结果可见，损伤后7d依托咪酯能显著增加视神经横断损伤大鼠Fluorogold逆行标记的视网膜神经节细胞数量，在损伤后12h能够显著降低视网膜中的一氧化氮和丙二醛水平，增加谷胱甘肽水平；(3) 结果说明，依托咪酯可通过抗氧化应激反应来保护视神经切断后损伤的视网膜神经节细胞。实验于2018年3月5日经中国第四军医大学动物伦理委员会批准，批准号20180305。

orcid: 0000-0001-7344-548X (Si-Wei You)

关键词: 依托咪酯, 视网膜神经节细胞, 视神经横断, 抗氧化应激, 一氧化氮, 丙二醛, 谷胱甘肽, 神经再生

Abstract:

Our previous studies revealed that etomidate, a non-barbiturate intravenous anesthetic agent, has protective effects on retinal ganglion cells within 7 days after optic nerve transection. Whether this process is related to anti-oxidative stress is not clear. To reveal its mechanism, we established the optic nerve transection injury model by transecting 1 mm behind the left eyeball of adult male Sprague-Dawley rats. The rats received an intraperitoneal injection of etomidate (4 mg/kg) once per day for 7 days. The results showed that etomidate significantly enhanced the number of retinal ganglion cells retrogradely labeled with Fluorogold at 7 days after optic nerve transection. Etomidate also significantly reduced the levels of nitric oxide and malonaldehyde in the retina and increased the level of glutathione at 12 hours after optic nerve transection. Thus, etomidate can protect retinal ganglion cells after optic nerve transection in adult rats by activating an anti-oxidative stress response. The study was approved by the Animal Ethics Committee at Air Force Medical University, China (approval No. 20180305) on March 5, 2018.

Key words: nerve regeneration, etomidate, retinal ganglion cells, optic nerve transection, anti-oxidative stress, nitric oxide, malonaldehyde, glutathione, neural regeneration