中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (5): 887-893.doi: 10.4103/1673-5374.268905

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

韩国红参可促进成年海马神经发生

  


  • 出版日期:2020-05-15 发布日期:2020-06-01
  • 基金资助:
    韩国教育部国家研究基金会（NRF）基础科学研究计划

Korean red ginseng promotes hippocampal neurogenesis in mice

Sun Ryu1, Hyongjun Jeon1, 2, Hee-Young Kim1, Sungtae Koo1, 2, Seungtae Kim1, 2   

  1. 1 Korean Medicine Research Center for Healthy Aging, Pusan National University, 49 Busandaehak-ro, Mulgeum-eup, Yangsan-si,
    Gyeongsangnam-do 50612, Republic of Korea
    2 Department of Korean Medical Science, School of Korean Medicine, Pusan National University, 49 Busandaehak-ro, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea
  • Online:2020-05-15 Published:2020-06-01
  • Contact: Seungtae Kim, PhD,kimst@pusan.ac.kr.
  • Supported by:
    This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. NRF-2016R1D1A3B03930920 to SK) and the MSIP (No. NRF-2014R1A5A2009936 to SK).

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摘要：

海马神经发生在认知能力中起主要作用,包括学习和记忆功能.韩国红参长期以来被称为药用植物,具有改善学习和记忆的潜力,但确切作用机制仍不明确.为了评估韩国红参是否能够促进成体海马的神经发生,实验给予8周龄雄性C57BL/6小鼠腹腔注射50mg/kg 5-溴脱氧尿嘧啶核苷(BrdU)，并喂饲100mg/kg 韩国红参或生理盐水，每天1次，持续14d。爬杆实验、平衡疲劳转动棒实验和Morris水迷宫测试评估显示，经过韩国红参干预的小鼠行为、学习和记忆功能均明显改善。海马齿状回BrdU和BrdU/双皮质素（神经元前体细胞和未成熟神经元的标记物）阳性细胞数量明显增多，海马细胞分化的标志物增殖细胞核抗原、大脑多巴胺神经营养因子和睫状神经营养因子基因表达明显上调。说明韩国红参可增强海马神经发生,改善学习和记忆功能。

orcid: 0000-0001-6033-5847 (Seungtae Kim)

关键词: 韩国红参, 神经发生, 海马, 学习, 记忆, 双皮质素, BrdU, 增殖细胞核抗原, 大脑多巴胺神经营养因子, 睫状神经营养因子

Abstract: Neurogenesis in the adult hippocampus plays a major role in cognitive ability of animals including learning and memory. Korean red ginseng (KRG) has long been known as a medicinal herb with the potential to improve learning and memory; however, the mechanisms are still elusive. Therefore, we evaluated whether KRG can promote cognitive function and enhance neurogenesis in the hippocampus. Eight-week-old male C57BL/6 mice received 50 mg/kg of 5-bromo-2′-deoxyuridine (BrdU) intraperitoneally and 100 mg/kg of KRG or vehicle orally once a day for 14 days. Pole, Rotarod and Morris water maze tests were performed and the brains were collected after the last behavioral test. Changes in the numbers of BrdU- and BrdU/ doublecortin (DCX; a marker for neuronal precursor cells and immature neurons)-positive cells in the dentate gyrus and the gene expression of proliferating cell nuclear antigen (a marker for cell differentiation), cerebral dopamine neurotrophic factor and ciliary neurotrophic factor in the hippocampus were then investigated. KRG-treated mice came down the pole significantly faster and stood on the rotarod longer than vehicle-treated mice. The Morris water maze test showed that KRG administration enhanced the learning and memory abilities significantly. KRG also significantly increased BrdU- and BrdU/DCX-positive cells in the dentate gyrus as well as the proliferating cell nuclear antigen, cerebral dopamine neurotrophic factor and ciliary neurotrophic factor mRNA expression levels in the hippocampus compared to vehicle. Administration of KRG promotes learning and memory abilities, possibly by enhancing hippocampal neurogenesis. This study was approved by the Pusan National University Institutional Animal Care and Use Committee (approval No. PNU-2016-1071) on January 19, 2016.

Key words: bromodeoxyuridine, cerebral dopamine neurotrophic factor, ciliary neurotrophic factor, doublecortin, ginseng, hippocampus, Korean red ginseng, learning, memory, neurogenesis, proliferating cell nuclear antigen, red ginseng