中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (8): 806-814.doi: 10.4103/1673-5374.131595

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

沉默Nogo受体基因的骨髓间充质干细胞修复脊髓损伤

  

  • 收稿日期:2014-02-08 出版日期:2014-04-25 发布日期:2014-04-25

Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

Zhiyuan Li 1, Zhanxiu Zhang 1, Lili Zhao 1, Hui Li 1, Suxia Wang 1, Yong Shen 2   

  1. 1 Department of Joint Orthopedics, Hebei Provincial Xingtai People’s Hospital, Xingtai, Hebei Province, China
    2 Department of Spinal Orthopedics, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
  • Received:2014-02-08 Online:2014-04-25 Published:2014-04-25
  • Contact: Zhiyuan Li, Department of Joint Orthopedics, Hebei Provincial Xingtai People’s Hospital, Xingtai, Hebei Province, China, myarcher@126.com.

摘要:

我们假设利用RNA干扰的方法沉默间充质干细胞中的Nogo受体基因能够改善移植修复脊髓损伤模型大鼠的效果。实验发现,干细胞移植2周后,与静脉移植未进行基因沉默转染的骨髓间充质干细胞组相比,转染siRNA沉默Nogo受体基因骨髓间充质干细胞移植的损伤脊髓组织中神经元和BrdU阳性细胞数量明显增加;4周后,模型大鼠行为能力明显增强;8周后,模型大鼠脊髓组织中HRP阳性神经纤维数量增加,出现新生的无髓及有髓神经纤维。说明沉默Nogo受体基因后的骨髓间充质干细胞移植对大鼠脊髓损伤有明显修复作用。

关键词: 神经再生, 脊髓损伤, 骨髓间充质干细胞, Nogo受体, RNA干扰, HRP, BrdU, 基因沉默

Abstract:

We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model          group. After 4 weeks, behavioral performance was significantly enhanced in the model group. After 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated nerve fibers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

Key words: nerve regeneration, spinal cord injury, bone marrow mesenchymal stem cells, Nogo-66 receptor, RNA interference, horseradish peroxidase, BrdU, gene silencing, neural regeneration