中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (2): 378-385.doi: 10.4103/1673-5374.317982

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

脊髓损伤大鼠脑内星形胶质细胞和小胶质细胞标志物表达的快速变化

  

  • 出版日期:2022-02-15 发布日期:2021-10-08

Rapid GFAP and Iba1 expression changes in the female rat brain following spinal cord injury

Mawj Mandwie1, Jordan A. Piper1, Catherine A. Gorrie2, Kevin A. Keay3, Giuseppe Musumeci4, Ghaith Al-Badri1, Alessandro Castorina1, 3, *#br#   

  1. 1Laboratory of Cellular and Molecular Neuroscience (LCMN), School of Life Science, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia; 2Neural Injury Research Unit, School of Life Science, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia; 3School of Medical Sciences (Neuroscience), The University of Sydney, Sydney, NSW, Australia; 4Department of Biomedical and Biotechnological Sciences, Anatomy, Histology and Movement Sciences Section, School of Medicine, University of Catania, Catania, Italy 
  • Online:2022-02-15 Published:2021-10-08
  • Contact: Alessandro Castorina, PhD, Alessandro.Castorina@UTS.edu.au.
  • Supported by:
    The University of Technology Sydney (UTS) Start-up Research Grant 2018 funded this study (to AC).

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摘要：

有证据表明，脊髓损伤（SCI）后会出现胶质细胞的快速变化。实验拟观察脊髓损伤后24h内大鼠脑内星形胶质细胞和小胶质细胞的特异性标志物胶质纤维酸性蛋白（GFAP）和离子钙接头蛋白分子1（Iba1）的表达。实验中，雌性SD大鼠中的一半建立轻度脊髓挫伤模型，另一半接受假手术。术后24h收集大鼠的杏仁核、灰质周围、前额叶皮质、下丘脑、侧丘脑、海马（背侧和腹侧）。以实时定量聚合酶链反应（qPCR）和免疫印迹检测胶质纤维酸性蛋白和离子钙接头蛋白分子1的mRNA和蛋白水平。在脊髓损伤大鼠中，胶质纤维酸性蛋白mRNA和蛋白的表达在杏仁核和下丘脑中增加，而在丘脑和背侧海马中表达下降。离子钙接头蛋白分子1 mRNA和蛋白表达仅在背侧和腹侧海马中显著减少。这些结果表明，早在脊髓损伤后24h，大鼠脑区的胶质纤维酸性蛋白和离子钙接头蛋白分子1基因和蛋白水平就有特异性改变。

https://orcid.org/0000-0001-7037-759X (Alessandro Castorina)

Abstract: Evidence suggests that rapid changes to supporting glia may predispose individuals with spinal cord injury (SCI) to such comorbidities. Here, we interrogated the expression of astrocyte- and microglial-specific markers glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) in the rat brain in the first 24 hours following SCI. Female Sprague-Dawley rats underwent thoracic laminectomy; half of the rats received a mild contusion injury at the level of the T10 vertebral body (SCI group), the other half did not (Sham group). Twenty-four hours post-surgery the amygdala, periaqueductal grey, prefrontal cortex, hypothalamus, lateral thalamus, hippocampus (dorsal and ventral) in rats were collected. GFAP and Iba1 mRNA and protein levels were measured by real-time quantitative polymerase chain reaction and Western blot. In SCI rats, GFAP mRNA and protein expression increased in the amygdala and hypothalamus. In contrast, gene and protein expression decreased in the thalamus and dorsal hippocampus. Interestingly, Iba1 transcripts and proteins were significantly diminished only in the dorsal and ventral hippocampus, where gene expression diminished. These findings demonstrate that as early as 24 hours post-SCI there are region-specific disruptions of GFAP and Iba1 transcript and protein levels in higher brain regions. All procedures were approved by the University of Technology Sydney Institutional Animal Care and Ethics Committee (UTS ACEC13-0069).

Key words: affective disorders, astrocytes, glial fibrillary acidic protein, ionized calcium binding adaptor molecule 1, memory, microglia, neurotrauma, spinal cord injury 

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