中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (19): 1743-1755.doi: 10.3969/j.issn.1673-5374.2013.19.002

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

中药复方脑必通治疗缺血性脑损伤的理论依据

  

  • 收稿日期:2013-02-10 修回日期:2013-04-25 出版日期:2013-07-05 发布日期:2013-07-05

Evidence for a therapeutic effect of Braintone on ischemic brain damage

Yuanyuan Qin1, 2, Yu Luo1, Weiwei Gu1, Lei Yang1, Xikun Shen2, Zhenlun Gu1, Huiling Zhang1, Xiumei Gao3   

  1. 1 Department of Pharmacology and Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Suzhou Institute of Chinese Meteria Medica, Soochow University, Suzhou 215123, Jiangsu Province, China
    2 Department of Pharmacy, Suzhou Hospital of Traditional Chinese Medicine, Suzhou 215009, Jiangsu Province, China
    3 Department of Anesthesiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2013-02-10 Revised:2013-04-25 Online:2013-07-05 Published:2013-07-05
  • Contact: Huiling Zhang, M.D., Ph.D., Professor, Department of Pharmacology and Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Suzhou Institute of Chinese Meteria Medica, Soochow University, Suzhou 215123, Jiangsu Province, China, huilingzhang07@ hotmail.com. Xiumei Gao, Master, Chief physician, Department of Anesthesiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China, gxmbj22@163.com.
  • About author:Yuanyuan Qin, Master, Pharmacist. Yuanyuan Qin and Yu Luo contributed equally to this work.

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摘要：

课题组创新性的结合了体内和体外实验进行，观察由红景天、三七、银杏叶和川芎四味中药精华组成的中成药脑必通的神经保护作用及相关分子机制。体内实验结果显示，连续7 d灌胃87.5，175，350 mg/kg脑必通预处理后，永久性大脑中动脉阻塞模型大鼠的脑梗死体积减小，脑含水量降低，神经功能明显改善。Western blot结果显示，模型大鼠缺血皮质低氧诱导因子1α、血红素加氧酶1 和血管内皮生长因子蛋白的表达，以350 mg/kg脑必通的作用最显著。体外实验中，分别向大鼠灌胃440，880，1760 mg/kg脑必通制备含药血清，并用其预处理人脐静脉内皮细胞24 h后进行糖氧剥夺干预，结果显示，人脐静脉内皮细胞损伤减轻。Real-time PCR及western blot结果显示，人脐静脉内皮细胞缺氧诱导因子1α mRNA及蛋白、血管内皮生长因子mRNA和血红素加氧酶1蛋白的表达提供，以1760mg/kg脑必通灌胃制备的含药血清作用最显著。作者认为，复方中药脑必通提高缺血区血管内皮细胞低氧诱导因子1α，血红素加氧酶1和血管内皮生长因子的表达是发挥其对缺血性脑损伤保护作用的物质基础。

关键词: 神经再生, 中医药, 脑必通, 缺血性脑损伤, 糖氧剥夺, 内皮细胞, 低氧诱导因子1α, 血管内皮生长因子, 血红素加氧酶1, 图片文章, 基金资助文章

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