Changes in secretory pathway Ca2+ -ATPase 2 following focal cerebral ischemia/reperfusion injury

doi:10.3969/j.issn.1673-5374.2013.01.010

Abstract

Abstract:

This study aimed to investigate changes in secretory pathway Ca²⁺ -ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca²⁺ -ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral artery occlusion method. Immunohistochemistry and reverse transcription-PCR assay results showed that compared with the control group, the expression of secretory pathway Ca²⁺ -ATPase 2 protein and mRNA in the cerebral cortex and hippocampus of male rats did not significantly change during the ischemic period. However, secretory pathway Ca²⁺ -ATPase 2 protein and mRNA expression reduced gradually at 1, 3, and 24 hours during the reperfusion period. Our experimental findings indicate that levels of secretory pathway Ca²⁺ -ATPase 2 protein and mRNA expression in brain tissue change in response to cerebral ischemia/reperfusion injury.

Key words: neural regeneration, brain injury, cerebral infarction, secretory pathway Ca²⁺ -ATPase 2, Golgi apparatus, Ca²⁺ oscillations, manganese, focal cerebral ischemia, oxidative damage, Ca²⁺ -ATPase, grants-supported paper, photographs-containing paper, neuroregeneration

Tonglin Lu, Zhiping Hu, Liuwang Zeng, Zheng Jiang. Changes in secretory pathway Ca²⁺ -ATPase 2 following focal cerebral ischemia/reperfusion injury[J]. Neural Regeneration Research, 2013, 8(1): 76-82.

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Changes in secretory pathway Ca²⁺ -ATPase 2 following focal cerebral ischemia/reperfusion injury

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