Chloride channel blocker 4,4- diisothiocyanatostilbene-2,2’-disulfonic acid inhibits nitric oxide-induced apoptosis in cultured rat hippocampal neurons

doi:10.3969/j.issn.1673-5374.2013.02.003

Abstract

Abstract:

Apoptosis in cultured rat hippocampal neurons was induced using the nitric oxide donor 3-morpholinosydnonimine, and cells were treated with the chloride channel blocker, 4,4- diisothiocyanatostilbene-2,2’-disulfonic acid. Results showed that the survival rate of neurons was significantly increased after treatment with 4,4-diisothiocyanatostilbene-2,2’-disulfonic acid, and the rate of apoptosis decreased. In addition, the expression of the apoptosis-related proteins poly(adenosine diphosphate-ribose)polymerase-1 and apoptosis-inducing factor were significantly reduced. Our experimental findings indicate that the chloride channel blocker 4,4- diisothiocyanatostilbene-2,2’-disulfonic acid can antagonize apoptotic cell death of hippocampal neurons by inhibiting the expression of the apoptosis-related proteins poly(adenosine diphosphate-ribose)polymerase-1 and apoptosis-inducing factor.

Key words: neural regeneration, brain injury, chloride channel, 3-morpholinosydnonimine, hippocampus, poly(adenosine diphosphate-ribose)polymerase-1, apoptosis inducing factor, neuronal apoptosis, grants-supported paper, photographs-containing paper, neuroregeneration

Jinbao Yin, Lijuan Xu, Shuling Zhang, Yuanyin Zheng, Zhichao Zhong, Hongling Fan, Xi Li, Quanzhong Chang. Chloride channel blocker 4,4- diisothiocyanatostilbene-2,2’-disulfonic acid inhibits nitric oxide-induced apoptosis in cultured rat hippocampal neurons[J]. Neural Regeneration Research, doi: 10.3969/j.issn.1673-5374.2013.02.003.

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