中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (8): 708-713.doi: 10.3969/j.issn.1673-5374.2013.08.005

• 原著:退行性病与再生 • 上一篇    下一篇

中国视神经脊髓炎患者血清中抗水通道蛋白4抗体的测定

  

  • 收稿日期:2012-05-17 修回日期:2012-11-15 出版日期:2013-03-15 发布日期:2013-03-15
  • 基金资助:

    意大利与中国双边科技合作项目

Detection of anti-aquaporin-4 autoantibodies in the sera of Chinese neuromyelitis optica patients

Miao Li1, 2, Weiheng Su3, Jie Wang2, Francesco Pisani4, Antonio Frigeri4, Tonghui Ma1   

  1. 1 Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, Jilin Province, China
    2 Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China
    3 National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, Jilin Province, China
    4 Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics (CEGBA), University of Bari, I-70126 Bari, Italy
  • Received:2012-05-17 Revised:2012-11-15 Online:2013-03-15 Published:2013-03-15
  • Contact: Tonghui Ma, M.D., Professor, Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, Jilin Province, China, math108@gmail.com.
  • About author:Miao Li★, Master, Attending physician, Lecturer.
  • Supported by:

    This work was supported by a grant from the China-Italy Bilateral Cooperation in Science and Technology Committee, No. PGR00011.

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摘要：

采集10例视神经脊髓炎患者、2例多发硬化患者和2例脊髓炎患者血清，将转染人AQP4-mCherry融合蛋白基因的V79细胞暴露在患者血清中，再利用免疫荧光法使与血清中水通道蛋白4抗体结合的V79细胞显影。结果显示此种方法只在视神经脊髓炎患者血清中检测出抗水通道蛋白4抗体，且在视神经脊髓炎患者中检测的灵敏度为90%，特异性为100%。通过倍比稀释法滴定视神经脊髓炎患者血清中抗水通道蛋白4抗体滴度，发现该滴度数值与患者的扩展的功能障碍评级呈正相关，提示以血清中抗水通道蛋白4抗体滴度的方法诊断中国视神经脊髓炎患者既灵敏又可靠，故检测可成为视神经脊髓炎诊断和治疗监测的重要手段。

关键词: 神经再生, 神经退行性变, 视神经脊髓炎, 多发性硬化症, 水通道蛋白4, 自身免疫性疾病, 自身抗体, 免疫荧光法, 功能障碍评级, 基金资助文章, 图片文章

Abstract:

In this study, we recruited 10 neuromyelitis optica patients, two multiple sclerosis patients and two myelitis patients. Chinese hamster lung fibroblast (V79) cells transfected with a human aquaporin-4-mCherry fusion protein gene were used to detect anti-aquaporin-4 antibody in neuromyelitis optica patient sera by immunofluorescence. Anti-aquaporin-4 autoantibody was stably detected by immunofluorescence in neuromyelitis optica patient sera exclusively. The sensitivity of the assay for neuromyelitis optica was 90% and the specificity for neuromyelitis optica was 100%. The anti-aquaporin-4 antibody titers in sera were tested with serial dilutions until the signal disappeared. A positive correlation was detected between Expanded Disability Status Scale scores and serum anti-aquaporin-4 antibody titers. The anti-aquaporin-4 antibody assay is highly sensitive and specific in the sera of Chinese neuromyelitis optica patients. Detection of aquaporin-4 autoantibody is important for the diagnosis and treatment of neuromyelitis optica.

Key words: neural regeneration, neurodegenerative diseases, neuromyelitis optica, multiple sclerosis, aquaporin-4, autoimmune disease, autoantibody, immunofluorescence, Expanded Disability Status Scale, grants-supported paper, photographs-containing paper, neuroregeneration