中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (9): 773-782.doi: 10.3969/j.issn.1673-5374.2013.09.001

• 原著:脑损伤修复保护与再生 •    下一篇

海巴戟和美金刚减轻脑水肿兔第四脑室周围组织损伤

  

  • 收稿日期:2012-01-23 修回日期:2013-02-24 出版日期:2013-03-25 发布日期:2013-03-25
  • 基金资助:

    土耳其科喀艾里大学医学院教育和研究基金资助项目(No. 2009/45).

Morinda citrifolia L. (noni) and memantine attenuate periventricular tissue injury of the fourth ventricle in hydrocephalic rabbits

Sibel Köktürk,1 Süreyya Ceylan2, Volkan Etus3, Nezih Yasa3, Savaş Ceylan3   

  1. 1 Department of Histology and Embriyology, Faculty of Medicine, Ordu University, Ordu, Turkey
    2 Department of Histology and Embriyology, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey
    3 Department of Neurosurgery, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey
  • Received:2012-01-23 Revised:2013-02-24 Online:2013-03-25 Published:2013-03-25
  • Contact: Sibel K?ktürk☆, M.D., Ph.D. Department of Histology and Embriyology, Faculty of Medicine, Ordu University, Cumhuriyet Campus, Ordu, Turkey, skokturk@mynet.com.
  • Supported by:

    This study was sponsored by a grant from the Education and Research Foundation of Faculty of Medicine, Kocaeli University, No. 2009/45.

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摘要：

为评价海巴戟（诺丽果）和美金刚对脑水肿引起的神经退行性变的保护效应。我们给予雄性成年新西兰兔小脑延髓池注射高岭土建立脑水肿动物模型，在此基础上连续2周每日腹腔注射20 mg/kg美金刚和每日喂饲海巴戟汁5 mL/kg。免疫组化方法检测兔第四脑室周围组织微管相关蛋白2和caspase-3，反映神经元退行性变情况。脑水肿兔第四脑室周围组织微管相关蛋白2表达明显减弱，而美金刚和海巴戟使微管相关蛋白2表达上升，且海巴戟的作用更为明显。海巴戟还可使脑水肿兔caspase-3阳性细胞数量明显减少，而美金刚则无此作用。说明海巴戟对脑水肿诱发的第四脑室周围组织的神经退行性变有明显抑制作用，且效果优于美金刚。

关键词: 神经退行性变, 中医药, 神经退行性变, 脑水肿, 诺丽果, 美金刚, 第四脑室, 脑室周围组织, 微管相关蛋白2, 细胞凋亡, 基金资助项目, 图片文章

Abstract:

This study was designed to evaluate the neuroprotective effects of Morinda citrifolia L. (Rubiaceae), commonly known as noni, and memantine (a N-methy-D-aspartate receptor inhibitor) on hydrocephalus-induced neurodegenerative disorders. Kaolin was injected into the cistern magna of male adult New Zealand rabbits to establish a hydrocephalus animal model. Memantine (20 mg/kg, intraperitoneally; memantine-treated group) or noni (5 mL/kg, intragastrically; noni-treated group) was administered daily for 2 weeks. Microtubule-associated protein-2 and caspase-3 immunohistochemistry were performed to detect neuronal degeneration and apoptosis in the periventricular tissue of the fourth ventricle of rabbits. Microtubule-associated protein-2 staining density was significantly decreased in the hydrocephalic group, while the staining density was significantly increased in the memantine- and noni-treated groups, especially in the noni-treated group. Noni treatment decreased the number of caspase-3-positive cells in rabbits with hydrocephalus, while memantine had no effect. These findings suggest that noni exhibits more obvious inhibitory effects on hydrocephalus-induced neurodegenerative disorders than memantine in periventricular tissue of the fourth ventricle.

Key words: neural regeneration, neurodegenerative disease, traditional Chinese medicine, hydrocephalus, Morinda citrifolia L. (noni), memantine, fourth ventricle, periventricular tissue, microtubule-associated protein-2, caspase-3, apoptosis, grants-supported paper, photographs-containing paper, neuroregeneration