中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (11): 983-990.doi: 10.3969/j.issn.1673-5374.2013.11.003

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

牛痘疫苗致炎提取物减轻孕鼠鞘内注射麻醉剂的脊神经毒性

  

  • 收稿日期:2012-12-02 修回日期:2013-02-09 出版日期:2013-04-15 发布日期:2013-04-15

Extracts from rabbit skin inflamed by the vaccinia virus attenuate bupivacaine-induced spinal neurotoxicity in pregnant rats

Rui Cui1, 2, Shiyuan Xu1, Liang Wang2, Hongyi Lei1, Qingxiang Cai1, Hongfei Zhang1, Dongmei Wang1   

  1. 1 Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China
    2 Department of Anesthesiology, Shenzhen Maternity & Child Healthcare Hospital, Southern Medical University, Shenzhen 518028, Guangdong Province, China
  • Received:2012-12-02 Revised:2013-02-09 Online:2013-04-15 Published:2013-04-15
  • Contact: Shiyuan Xu, Master, Professor, Doctoral supervisor, Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China, xushiyuan355@ yahoo.com.cn.
  • About author:Rui Cui☆, M.D., Associate chief physician.

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摘要：

牛痘疫苗致炎兔皮提取物注射液具有减轻疼痛，促进神经损伤修复的作用。实验于孕鼠鞘内注入布比卡因前后分别连续腹腔注射牛痘疫苗致炎兔皮提取物注射液3 d和4 d。鞘内注射布比卡因后大鼠痛阈检测结果显示，牛痘疫苗致炎兔皮提取物注射液预处理组大鼠热辐射甩尾反应潜伏期的最大效应百分比在鞘内注射布比卡因后1 d达高峰，随后呈下降趋势，而布比卡因组大鼠的最大效应百分比在鞘内注射布比卡因后持续增高。组织学观察显示，鞘内注入布比卡因后4 d，相比布比卡因组，牛痘疫苗致炎兔皮提取物注射液预处理组大鼠脊髓背根节中萎缩细胞、空泡化细胞，凋亡细胞和caspase-9阳性细胞数量均明显减少。说明预先给予牛痘疫苗致炎兔皮提取物注射液可以减轻孕鼠鞘内注入布比卡因的脊神经毒性，其机制可能与抑制caspase-9蛋白表达，抗神经细胞凋亡有关。

关键词: 神经再生, 牛痘疫苗, 兔, 提取物, 妊娠, 布比卡因, 神经毒性, caspase-9, 凋亡, 局部麻醉药, 预处理, 基金资助文章

Abstract:

Extracts from rabbit skin inflamed by the vaccinia virus can relieve pain and promote repair of nerve injury. The present study intraperitoneally injected extracts from rabbit skin inflamed by the vaccinia virus for 3 and 4 days prior to and following intrathecal injection of bupivacaine into pregnant rats. The pain threshold test after bupivacaine injection showed that the maximum possible effect of tail-flick latency peaked 1 day after intrathecal injection of bupivacaine in the extract-pretreatment group, and gradually decreased, while the maximum possible effect in the bupivacaine group continued to increase after intrathecal injection of bupivacaine. Histological observation showed that after 4 days of intrathecal injection of bupivacaine, the number of shrunken, vacuolated, apoptotic and caspase-9-positive cells in the dorsal root ganglion in the extract-pretreatment group was significantly reduced compared with the bupivacaine group. These findings indicate that extracts from rabbit skin inflamed by the vaccinia virus can attenuate neurotoxicity induced by intrathecal injection of bupivacaine in pregnant rats, possibly by inhibiting caspase-9 protein expression and suppressing nerve cell apoptosis.

Key words: neural regeneration, peripheral nerve injury, vaccinia virus, extract, bupivacaine, neurotoxicity, caspase-9, apoptosis, local anesthetic, grants-supported paper, neuroregeneration