中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1061-1070.doi: 10.3969/j.issn.1673-5374.2013.12.001

• 原著:退行性病与再生 •    下一篇

中药天麻治疗阿尔茨海默病的潜力

  

  • 收稿日期:2012-11-06 修回日期:2013-03-06 出版日期:2013-04-25 发布日期:2013-04-25

Therapeutic potential of Gastrodia elata Blume for the treatment of Alzheimer’s disease

Guang-Biao Huang1, Tong Zhao1, Sushma Shrestha Muna1, Hong-Mei Jin1, Jong-Il Park1, Kyu-Sik Jo2, Bo-Hee Lee2, Soo-Wan Chae3, Sun-Young Kim3, Soo-Hyun Park3, Eun-Ock Park3, Eun-Kyung Choi3, Young-Chul Chung1, 4   

  1. 1 Department of Psychiatry, Chonbuk National University Medical School, Jeonju 561-756, Republic of Korea
    2 Muju Chunma Native Local Industrial Center, Muju 568-844, Republic of Korea
    3 Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju 561-756, Republic of Korea
    4 Department of Pharmacology, Chonbuk National University Medical School, Jeonju 561-756, Republic of Korea
    5 Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 561-756, Republic of Korea
  • Received:2012-11-06 Revised:2013-03-06 Online:2013-04-25 Published:2013-04-25
  • Contact: Young-Chul Chung, M.D., Professor, Department of Psychiatry, Chonbuk National University Medical School, Keumam Dong 634-18, Jeonju 561-712, Republic of Korea, chungyc@jbnu.ac.kr.
  • About author:Guang-Biao Huang☆, M.D., Researcher. Guang-Biao Huang and Tong Zhao contributed equally to this paper.
  • Supported by:

    韩国茂朱天麻天然地方工业中心资助

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摘要：

研究证实，中草药天麻可减少淀粉样beta蛋白诱导的细胞凋亡。为研究天麻对阿尔茨海默病的可能治疗作用，我们将淀粉样beta蛋白25-35注射于大鼠双侧海马，建立阿尔茨海默病大鼠模型，然后连续52d每日灌胃500 或1000mg/kg天麻。治疗结束后，Morris水迷宫试验评估显示阿尔茨海默病大鼠空间记忆功能明显改善，刚果红染色显示阿尔茨海默病大鼠海马淀粉样沉积明显减少，Western blotting和Ellman法检测显示阿尔茨海默病大鼠额前皮质、内侧隔核和海马胆碱乙酰基转移酶表达上升，乙酰胆碱酯酶活性明显下降。说明长期应用天麻治疗阿尔茨海默病是很有改善症状潜力的方法。

关键词: 神经再生, 中医药, 天麻, 阿尔茨海默病, 老年痴呆, 水迷宫试验, 胆碱乙酰基转移酶, 乙酰胆碱酯酶, 基金资助文章

Abstract:

Several studies have demonstrated that the Chinese herb Gastrodia elata Blume can protect against amyloid beta-peptide (Aβ)-induced cell death. To investigate the possible therapeutic effects of Gastrodia elata Blume on Alzheimer’s disease, we established a rat model of Alzheimer’s disease by injecting Aβ25-35 into bilateral hippocampi. These rats were intragastrically administered 500 or 1 000 mg/kg Gastrodia elata Blume per day for 52 consecutive days. Morris water maze tests showed that Gastrodia elata Blume treatment significantly improved the spatial memory of Alzheimer’s disease rats. Congo red staining revealed that Gastrodia elata Blume significantly reduced the number of amyloid deposits in the hippocampus of these rats. Western blot analysis showed that choline acetyltransferase expression in the medial septum and hippocampus was significantly increased by the treatment of Gastrodia elata Blume, while Ellman method showed significant decrease in the activity of acetylcholinesterase in all three regions (prefrontal cortex, medial septum and hippocampus). These findings suggest that long-term administration of Gastrodia elata Blume has therapeutic potential for Alzheimer’s disease.

Key words: neural regeneration, neuroprotective effect, Traditional Chinese medicine, Gastrodia elata Blume, Alzheimer’s disease, Morris water maze test, choline acetyltransferase, acetylcholinesterase, grants-supported paper, neuroregeneration