中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (5): 893-898.doi: 10.4103/1673-5374.297088

• 原著:退行性病与再生 • 上一篇    下一篇

牙髓干细胞移植治疗阿尔茨海默病的潜力

  

  • 出版日期:2021-05-15 发布日期:2020-12-29
  • 基金资助:

    哈尔滨医科大学于卫汉杰出青年基金(002000013

Therapeutic potential of dental pulp stem cell transplantation in a rat model of Alzheimer’s disease

Xue-Mei Zhang1, #, Yuan-Jiao Ouyang1, #, Bing-Qian Yu2, Wei Li1, Mei-Yu Yu1, Jin-Yue Li1, Zhuo-Min Jiao1, Dan Yang1, Na Li3, Ying Shi1, Yun-Yun Xu1, Zhi-Jun He1, Duo Wang1, Hui Yue1, Jin Fu1, *#br#   

  1. 1 Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China;  2 Department of Neurology, Ningbo Hangzhou Bay Hospital, Ningbo, Zhejiang Province, China;  3 Department of Neurology, the First Hospital of Qiqihar, Qiqihar, Heilongjiang Province, China
  • Online:2021-05-15 Published:2020-12-29
  • Contact: Jin Fu, PhD, MD, fujin198@126.com.
  • Supported by:
    This study was supported by Yu Weihan Fund for Distinguished Young Scholars of Harbin Medical University of China, No. 002000013 (to XMZ).

摘要:

牙髓干细胞是一种来源于牙髓组织的间充质干细胞,由于其发源于胚胎神经嵴,且具有神经源性分化潜能,还能分泌多种神经营养因子,因此较其他类型的干细胞,在治疗神经系统疾病方面更具潜能。但是尚未见有关牙髓干细胞移植治疗阿尔茨海默病的更多研究报道。实验设计给大鼠海马定向注射β-淀粉样蛋白1-42建立阿尔茨海默病模型大鼠,14d后向海马注射5 × 106 牙髓干细胞。免疫组化以及Western blot结果显示,牙髓干细胞移植后,海马中神经细胞相关蛋白(双皮质素、神经元核蛋白、神经丝蛋白)的表达明显增加,而β-淀粉样蛋白含量减少,且大鼠认知行为出现明显改善。表明牙髓干细胞移植可通过影响神经元相关蛋白的分泌,改善认知功能,从而起到治疗阿尔茨海默病的效果。动物实验于2017221日经哈尔滨医科大学实验动物伦理委员会批准,批准号KY2017-132

https://orcid.org/0000-0001-8425-8129 (Jin Fu)

关键词: 中枢神经系统, 脑, 阿尔茨海默病, 模型, 大鼠, 体内, 干细胞, 牙髓干细胞, 移植

Abstract: Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest. They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells because of their neurogenic differentiation capability and their ability to secrete multiple neurotrophic factors. Few studies have reported Alzheimer’s disease treatment using dental pulp stem cells. Rat models of Alzheimer’s disease were established by injecting amyloid-β1–42 into the hippocampus. Fourteen days later, 5 × 106 dental pulp stem cells were injected into the hippocampus. Immunohistochemistry and western blot assays showed that dental pulp stem cell transplantation increased the expression of neuron-related doublecortin, NeuN, and neurofilament 200 in the hippocampus, while the expression of amyloid-β was decreased. Moreover, cognitive and behavioral abilities were improved. These findings indicate that dental pulp stem cell transplantation in rats can improve cognitive function by regulating the secretion of neuron-related proteins, which indicates a potential therapeutic effect for Alzheimer’s disease. This study was approved by the Animal Ethics Committee of Harbin Medical University, China (approval No. KY2017-132) on February 21, 2017.

Key words: Alzheimer’s disease, brain, central nervous system, dental pulp stem cell, in vivo, model, rat, stem cells, transplantation