中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1091-1102.doi: 10.3969/j.issn.1673-5374.2013.12.004

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

加味四逆散促进应激损伤海马神经发生

  

  • 收稿日期:2012-11-27 修回日期:2013-03-06 出版日期:2013-04-25 发布日期:2013-04-25

Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage

Lili Wu1, Chuanlian Ran2, Shukao Liu3, Lizhen Liao4, Yanling Chen4, Hualei Guo4, Weikang Wu4, Can Yan1   

  1. 1 Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, China
    2 Department of Traditional Chinese Medicine, Luzhou Medicine College, Luzhou 646000, Sichuang Province, China
    3 Institute of Pharmaceutical Research, Yangtze River Pharmaceutical Group Company Limited, Taizhou 225321, Jiangsu Province, China
    4 Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2012-11-27 Revised:2013-03-06 Online:2013-04-25 Published:2013-04-25
  • Contact: Weikang Wu, M.D., Ph.D., Associate professor, Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China, w2004wk@yahoo.com.cn. Can Yan, M.D., Ph.D., Professor, Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, China, yancan999@yahoo.com.cn.
  • About author:Lili Wu☆, M.D., Ph.D., Associate professor.

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摘要：

作者前期的研究证实，传统中药加味四逆散具有确切的抗抑郁效应，能抑制应激性抑郁症时下丘脑-垂体-肾上腺轴的亢进。实验采用体外无血清培养大鼠海马神经前体细胞，以120μM皮质酮培养模拟应激损伤细胞模型，然后用10%加味四逆散含药血清及皮质酮拮抗剂RU38486干预。MTT检测显示，加味四逆散含药血清和RU38486可增加皮质酮损伤海马神经前体细胞的增殖率。免疫荧光检测显示，经皮质酮拮抗剂RU38486或加味四逆散含药血清干预后，皮质酮损伤的海马神经前体细胞中BrdU/TUNEL比值明显升高，且分化的神经胶质样细胞和神经元样细胞凋亡减少。提示加味四逆散能促进应激条件下的海马神经发生。

关键词: 神经再生, 中医药, 加味四逆散, 皮质酮, 海马神经前体细胞, 分化, 增值, 凋亡, 基金资助文章

Abstract:

The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.

Key words: neural regeneration, traditional Chinese medicine, Jiaweisinisan, corticosterone, hippocampal neural precursor cells, proliferation, differentiation, apoptosis, grants-supported paper, neuroregeneration