中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1122-1131.doi: 10.3969/j.issn.1673-5374.2013.12.007

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

保持星形胶质细胞结构完整性的最佳电针频率

  

  • 收稿日期:2012-12-28 修回日期:2013-03-19 出版日期:2013-04-25 发布日期:2013-04-25

Optimal electroacupuncture frequency for maintaining astrocyte structural integrity in cerebral ischemia

Yicai Xiao1, Xingui Wu1, Xiangfa Deng2, Liping Huang1, Yuancheng Zhou1, Xuejie Yang1   

  1. 1 Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
    2 Department of Anatomy, Faculty of Preclinical Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2012-12-28 Revised:2013-03-19 Online:2013-04-25 Published:2013-04-25
  • Contact: Xingui Wu, Ph.D., Chief physician, Master’s supervisor, Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China, wxingui2000@yahoo.com.cn
  • About author:Yicai Xiao★, Master.
  • Supported by:

    广西中医药管理局立项课题(No.GZZC0962)

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摘要：

星形胶质细胞可决定缺血性脑损伤神经元的存活。电针作为脑缺血的干预措施之一，此时，选择何种频率的电针刺激会影响到脑组织星形胶质细胞的结构完整性？在脑缺血治疗中电针频率可影响治疗效果。鉴于此，实验采用线栓法制成大脑中动脉闭塞大鼠模型，分别用15，30，100 Hz频率电针进行干预，以行为学检测、免疫组织化学染色及电镜观察检测结果显示，与模型组及100 Hz电针组相比，15，30 Hz电针组Longa评分较低，脑组织星形胶质细胞标志物--胶质纤维酸性蛋白表达较高，梗死灶边缘组织星形胶质细胞超微结构损伤程度减轻。说明15，30 Hz频率电针干预可较好的维持星形胶质细胞的结构完整性，对脑缺血损伤发挥保护作用。星形胶质细胞结构完整性可能是针刺产生脑缺血耐受的作用机制之一。

关键词: 神经再生, 针灸, 脑损伤, 电针频率, 局灶性脑缺血, 胶质纤维酸性蛋白, 星形胶质细胞, 神经保护, 脑缺血耐受, 电镜, 基金资助文章

Abstract:

The astrocyte is a critical regulator of neuronal survival after ischemic brain injury. Electroacupuncture may be an effective therapy for cerebral ischemia, as electroacupuncture frequency can affect the structural integrity of astrocytes. In this study, a rat model of middle cerebral artery occlusion established using the modified thread embolism method was treated with electroacupuncture of the bilateral Quchi (LI11) and Zusanli (ST36) at 15, 30, and 100 Hz frequencies. Behavioral testing, immunohistochemistry and electron microscopy were used to explore the effect of these electroacupuncture frequencies used on maintaining the structural integrity of ischemic brain tissue. Compared with the model and 100 Hz electroacupuncture groups, the 15 and 30 Hz electroacupuncture groups displayed decreased neurological deficit scores, as evaluated by the “Longa” method, significantly increased glial fibrillary acidic protein expression, and alleviated ultrastructural damage of astrocytes at the edge of the infarct. Our experimental findings indicate that 15 and 30 Hz electroacupuncture intervention can favorably maintain the structural integrity of astrocytes and play a protective role in cerebral ischemic injury. Astrocyte structural integrity may be the mechanism underlying acupuncture production of ischemic tolerance.

Key words: neural regeneration, acupuncture and moxibustion, brain damage, electroacupuncture frequency, focal ischemia, glial fibrillary acidic protein, astrocytes, neuroprotection, cerebral ischemic tolerance, electron microscope, grants-supported paper, neuroregeneration