中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (23): 2134-2143.doi: 10.3969/j.issn.1673-5374.2013.23.003

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

急性脑梗死后血小板高尔基体的形态变化及其意义

  

  • 收稿日期:2013-03-20 修回日期:2013-06-01 出版日期:2013-08-15 发布日期:2013-08-15

Morphology of platelet Golgi apparatus and their significance after acute cerebral infarction

Wei Lu, Dong Xu, Ranran Tu, Zhiping Hu   

  1. Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
  • Received:2013-03-20 Revised:2013-06-01 Online:2013-08-15 Published:2013-08-15
  • Contact: Zhiping Hu, M.D., Chief physician, Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China, huzhipingxy@ gmail.com.
  • About author:Wei Lu, M.D., Associate chief physician.
  • Supported by:

    国家自然科学基金项目(81171239/H0914)；中南大学重点研究项目基金(No.2177-721500065)；湖南省财政厅教育支出项目 (No. 2010163).

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摘要：

采集20例急性脑梗死患者发病后第1，7，15天空腹肘静脉血，制备血小板悬液，另选取20名正常成人空腹肘静脉血作对照。透射电镜下见急性脑梗死后血小板高尔基体小管和囊泡明显增粗、膨大、排列欠规整；血小板内α颗粒明显减少，以脑梗死后1 d减少最明显。免疫电镜观察发现急性脑梗死后仅有少量的α颗粒聚集在高尔基体小管及囊泡附近。表明急性脑梗死后血小板高尔基体发生明显的形态变化并可能与血小板活化后合成和分泌功能增强有关。为研究高尔基体对血小板表达CD40L的影响，实验进一步应用高尔基体阻断剂布雷非德菌素-A阻断正常成人高尔基体功能，流式细胞检测显示，高尔基体功能被阻断后血小板CD40L表达显著降低，提示高尔基体可能参与了血小板CD40L的合成和转运。

关键词: 高尔基体, CD40L, α颗粒, 血小板, 超微结构, 脑梗死, 转运, 分泌, 布雷非德菌素-A, 神经再生

Abstract:

Blood samples were harvested from the antecubital vein of 20 fasting patients with acute cerebral infarction at 1, 7 and 15 days after onset to prepare blood platelet suspension. Fasting antecubital vein blood was collected from an additional 20 normal adults as controls. Under transmission elec-tron microscope, platelet Golgi tubules and vesicles became significantly thickened, enlarged, and irregular after acute cerebral infarction. Alpha granules in platelets significantly reduced in number, especially 1 day after cerebral infarction. Under immunoelectron microscopy, a few alpha granules aggregated around Golgi tubules and vesicles after infarction. These results suggested that platelet Golgi apparatus displayed significant morphological changes, which were possibly associated with enhanced synthetic and secretory functions of activated platelets after acute cerebral infarction. This study used Golgi apparatus blocking agent Brefeldin A to block Golgi apparatus in an aim to study the effects of Golgi apparatus on CD40L expression on the surface of activated platelets. Flow cytometry revealed that CD40L expression on activated platelet surfaces decreased significantly when Golgi apparatus was blocked, which indicated that Golgi apparatus participated in the syn-thesis and transport of CD40L to the platelet surface.

Key words: neural regeneration, brain injury, Golgi apparatus, CD40L, alpha granules, platelet, ultrastructure, cerebral infarction, transport, secretion, Brefeldin A, grants-supported paper, neuroregeneration