中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1645-1651.doi: 10.4103/1673-5374.303042

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

牙髓干细胞移植对血管性痴呆模型大鼠的治疗作用

  

  • 出版日期:2021-08-15 发布日期:2021-01-14
  • 基金资助:

    哈尔滨医科大学于维汉杰出青年基金(002000013

Therapeutic effects of dental pulp stem cells on vascular dementia in rat models

Xue-Mei Zhang#, Yang Sun#, Ying-Lian Zhou, Zhuo-Min Jiao, Dan Yang,  Yuan-Jiao Ouyang, Mei-Yu Yu, Jin-Yue Li, Wei Li, Duo Wang, Hui Yue, Jin Fu*   

  1. Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China
  • Online:2021-08-15 Published:2021-01-14
  • Contact: Jin Fu, PhD, MD, fujin@hrbmu.edu.cn.
  • Supported by:
    This study was supported by Yu Weihan Fund for Distinguished Young Scholars of Harbin Medical University of China, No. 002000013 (to XMZ).

摘要:

牙髓干细胞是一种成年干细胞,具有高增殖能力和多向分化潜能,但至今尚无牙髓干细胞治疗血管性痴呆的研究,此次实验以两血管闭塞建立血管性痴呆大鼠模型,30d后通过尾静脉注射2 × 107牙髓干细胞。70d时检测发现在血管性痴呆大鼠模型脑组织中可见迁移的牙髓干细胞,且其分化为神经元样细胞,同时脑组织中DCX,神经丝200和NeuN mRNA和蛋白表达增加,而神经胶质纤维酸性蛋白mRNA和蛋白表达减少。同时行为学测试也证实了牙髓干细胞移植可改善血管性痴呆大鼠的认知功能。表明牙髓干细胞移植可能通过旁分泌方式修复血管性痴呆大鼠的神经功能,对其产生治疗效应。实验于2017年在哈尔滨医科大学动物伦理委员会批准,批准号KY2017-132

https://orcid.org/0000-0001-7532-784X (Jin Fu)

关键词: 牙髓干细胞, 干细胞, 血管性痴呆, 大鼠, 动物模型, 移植, 旁分泌, 修复

Abstract: Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the present study, rat models of vascular dementia were established by two-vessel occlusion, and 30 days later, rats were injected with 2 × 107 dental pulp stem cells via the tail vein. At 70 days after vascular dementia induction, dental pulp stem cells had migrated to the brain tissue of rat vascular dementia models and differentiated into neuron-like cells. At the same time, doublecortin, neurofilament 200, and NeuN mRNA and protein expression levels in the brain tissue were increased, and glial fibrillary acidic protein mRNA and protein expression levels were decreased. Behavioral testing also revealed that dental pulp stem cell transplantation improved the cognitive function of rat vascular dementia models. These findings suggest that dental pulp stem cell transplantation is effective in treating vascular dementia possibly through a paracrine mechanism. The study was approved by the Animal Ethics Committee of Harbin Medical University (approval No. KY2017-132) in 2017.

Key words: animal model, dental pulp stem cells, paracrine, repair, stem cells, transplantation, vascular dementia