中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (25): 2350-2359.doi: 10.3969/j.issn.1673-5374.2013.25.006

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺治疗神经病理性痛的信号途径

  

  • 收稿日期:2013-06-26 修回日期:2013-06-26 出版日期:2013-09-05 发布日期:2013-09-05
  • 基金资助:

    中国博士后科学基金(20100480643)；上海市教育委员会科研创新项目 (2011JW13)

Molecular mechanisms underlying the effects of acupuncture on neuropathic pain

Ziyong Ju1, Huashun Cui2, Xianhui Guo1, Huayuan Yang1, Jinsen He1, Ke Wang3   

  1. 1 College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

    2 Department of Acupuncture, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

    3 Department of Cardiothoracic Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2013-06-26 Revised:2013-06-26 Online:2013-09-05 Published:2013-09-05
  • Contact: Jinsen He, M.D., Professor, College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China, hejinsen@citiz.net. Ke Wang, M.D., Department of Cardiothoracic Surgery,, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China, wangke8430@ 163.com.
  • About author:Ziyong Ju, M.D. Ziyong Ju and Huashun Cui contributed equally to this work.
  • Supported by:

    This study was supported by the China Postdoctoral Science Foundation, No. 20100480643; and the Program of Shanghai Municipal Education Commission, No. 2011JW13.

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摘要：

研究表明针灸治疗能有效改善神经病理性疼痛，然而其机制尚不完全明确。实验通过慢性压迫性损伤诱导大鼠神经病理性痛模型，采用临床针灸治疗中常用的电针刺激和手针刺激作为干预手段，观察电针和手针疗法对神经病理性痛的效应以及对脊髓背角EphrinBs/EphBs信号系统的影响。结果显示，电针和手针均能显著改善由慢性压迫性损伤造成的大鼠机械性痛觉过敏，电针效果更佳。实时荧光定量RT-PCR结果显示，模型大鼠脊髓背角EphrinB1/B3和EphB1/B2 mRNA表达显著增加，电针和手针干预可抑制EphrinB1 mRNA的高表达，提高EhpB3/B4 mRNA的表达。尤其是电针处理能够显著提高模型大鼠脊髓背角EphB3/B6和EphrinB3 mRNA的表达。Western blot结果显示，手针和电针均能显著上调模型大鼠脊髓背角EphrinB3蛋白的表达，以电针作用更显著。作者认为，针灸治疗神经病理性疼痛的作用在于改善了神经功能与激活EphrinBs/EphBs信号系统亚单位密切相关。

关键词: 神经再生, 针灸, 神经病理性疼痛, 周围神经损伤, 慢性压迫性损伤, 电针, 脊髓背角, ephrinBs/ephBs信号, 神经修复, 基金资助文章

Abstract:

Acupuncture has been used to treat neuropathic pain for a long time, but its mechanisms of action remain unknown. In this study, we observed the effects of electroacupuncture and manual acu-puncture on neuropathic pain and on ephrin-B/EphB signaling in rats models of chronic constriction injury-induced neuropathic pain. The results showed that manual acupuncture and electroacupuncture significantly reduced mechanical hypersensitivity following chronic constriction injury, especially electroacupuncture treatment. Real-time PCR results revealed that ephrin-B1/B3 and EphB1/B2 mRNA expression levels were significantly increased in the spinal dorsal horns of chronic constriction injury rats. Electroacupuncture and manual acupuncture suppressed the high expression of ephrin-B1 mRNA, and elevated EphB3/B4 mRNA expression. Electroacupuncture significantly enhanced the mRNA expression of ephrin-B3 and EphB3/B6 in the dorsal horns of neuropathic pain rats. Western blot results revealed that electroacupuncture in particular, and manual acupuncture, significantly up-regulated ephrin-B3 protein levels in rat spinal dorsal horns. The results of this study suggest that acupuncture could activate ephrin-B/EphB signaling in neu-ropathic pain rats and improve neurological function.

Key words: neural regeneration, acupuncture, neuropathic pain, chronic constriction injury, electroacupuncture, spinal dorsal horn, ephrin-B/EphB signaling, nerve repair, grants-supported paper, neuroregeneration