中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (28): 2597-2604.doi: 10.3969/j.issn.1673-5374.2013.28.001

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黄芩苷可抑制硫酸粘杆菌素诱导PC12细胞的凋亡

  

  • 收稿日期:2013-05-10 修回日期:2013-08-02 出版日期:2013-10-05 发布日期:2013-10-05

Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

Hong Jiang1, 2, Pengfei Lv3, Jichang Li1, Hongjun Wang2, Tiezhong Zhou2, Yingzi Liu2, Wei Lin1   

  1. 1 Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China
    2 Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
    3 Scientific Research Department, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Received:2013-05-10 Revised:2013-08-02 Online:2013-10-05 Published:2013-10-05
  • Contact: Jichang Li, M.D., Professor, Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China, lijichang828@sina.com. Hongjun Wang, M.D., Associate professor, Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China, lnwhj2012@163.com
  • About author:Hong Jiang, Studying for doctorate, Lecturer.

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摘要：

鉴于干燥的黄芩根中提取的黄酮类化合物黄芩苷可以有效抑制细胞凋亡，我们假设黄芩苷可抑制硫酸粘杆菌素诱导的神经元凋亡。实验首先以62.5–500 μg/mL硫酸粘杆菌素孵育PC12细胞24h，发现该剂量范围的硫酸粘杆菌素能够诱导PC12细胞凋亡，且细胞中Caspase-3、乳酸脱氢酶和自由基含量呈现剂量依赖性升高。然后将PC12细胞以25，50，100 μg/mL黄芩苷预处理，再经125μg/mL硫酸粘杆菌素诱导，此时细胞形态明显改观，细胞存活率增加，细胞中Caspase-3及乳酸脱氢酶和自由基含量显著降低。结果提示黄芩苷通过抑制自由基损伤、减少caspase-3表达和乳酸脱氢酶活性，抑制硫酸粘菌素诱导PC12细胞的凋亡，抑制硫酸粘杆菌素神经毒性的作用。

关键词: 神经再生, 中医药, 黄芩苷, 硫酸粘杆菌素, PC12细胞, 凋亡, Caspase-3, 乳酸脱氢酶, 基金资助文章, 图片文章

Abstract:

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5–500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, lac-tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhib-ited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.