中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (34): 3233-3240.doi: 10.3969/j.issn.1673-5374.2013.34.007

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

缺糖缺氧模型神经元体外转染Toll样受体3-siRNA的最优转染序列

  

  • 收稿日期:2013-08-20 修回日期:2013-08-20 出版日期:2013-12-05 发布日期:2013-12-05

Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA Determination of an optimal transfection sequence

Guiyun Cui, Xiaopeng Wang, Xinchun Ye, Jie Zu, Kun Zan, Fang Hua   

  1. Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • Received:2013-08-20 Revised:2013-08-20 Online:2013-12-05 Published:2013-12-05
  • Contact: Guiyun Cui, M.D., Associate professor, Chief physician, Master’s supervisor, Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China, cuiguiyun-js@ 163.com. Fang Hua, Ph.D., Professor, Doctoral supervisor, Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China, huafangxz @hotmail.com.
  • About author:Guiyun Cui and Xiaopeng Wang contributed equally to this paper.

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摘要：

课题组前期研究发现，大鼠脑缺血再灌注损伤过程中脑组织Toll样受体3蛋白表达上调。鉴于此，实验以体外原代培养大鼠胎鼠皮质神经元建立缺糖缺氧模型，模拟脑缺血再灌注损伤过程，将化学合成的3对Toll样受体3基因小干扰RNA序列Toll样受体3-1280，1724，418转染入缺糖缺氧模型的皮质神经元中，以期抑制Toll样受体3蛋白表达的上调。Western-blot检测结果表明，3对Toll样受体3基因小干扰RNA转染后，皮质神经元Toll样受体3蛋白表达量均减少，尤以Toll样受体3-1724组抑制TLR3蛋白表达效果最为显著。结果证实体外缺糖缺氧条件下Toll样受体3-1724-小干扰RNA序列为抑制皮质神经元Toll样受体3表达的最优转染序列。

中国神经再生研究（英文版）杂志出版内容重点： 脑损伤；脊髓损伤；周围神经损伤；帕金森；神经影像；神经再生

关键词: 神经再生, 脑损伤, Toll样受体3, 小干扰RNA, 原代神经元, 缺血再灌注损伤, 转染, 缺氧, 脂质体, 免疫炎症反应, RNA干扰, 基金资助文章