中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (2): 198-204.doi: 10.4103/1673-5374.125350

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

孤束核在艾灸预处理保护胃黏膜损伤中的作用

  

  • 收稿日期:2013-11-25 出版日期:2014-01-10 发布日期:2014-01-10

Role of the nucleus tractus solitarii in the protection of pre-moxibustion on gastric mucosal lesions

Liang Peng, Mi Liu, Xiaorong Chang, Zhou Yang, Shouxiang Yi, Jie Yan, Yan Peng   

  1. College of Acupuncture and Tuina, Hunan University of Chinese Medicine, Changsha, Hunan Province, China
  • Received:2013-11-25 Online:2014-01-10 Published:2014-01-10
  • Contact: Xiaorong Chang, College of Acupuncture and Tuina, Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China, xrchang1956@163.com.

摘要:

有研究表明,针刺的躯体感觉传入和内脏伤害性刺激传入可能在孤束核发生汇聚,并通过孤束核神经元的整合后影响内脏的功能。为了探讨艾灸预处理保护胃黏膜的神经机制,实验预先损毁大鼠双侧孤束核后进行足三里穴(ST36)艾灸预处理,再采用无水乙醇灌胃法损伤大鼠胃黏膜。形态学观察,酶联免疫吸附法和Western-blot法检测结果显示,艾灸预处理的大鼠损伤胃黏膜的表面破损及炎细胞浸润程度明显减轻,胃黏膜损伤指数降低,胃黏膜组织中表皮生长因子含量较高,生长抑素含量较低,热休克蛋白70表达较高,在此基础上预先用孤束核损毁干预可逆转上述现象。结果说明,艾灸足三里穴预处理对胃黏膜损伤有明显保护作用,孤束核损毁可明显抑制其保护效应,由此推断孤束核是艾灸减轻胃黏膜损伤的重要中枢神经信息调节部位。

关键词: 神经再生, 中医药, 艾灸, 孤束核, 胃黏膜损伤, 热休克蛋白70, 表皮生长因子, 生长抑素

Abstract:

Previous studies have shown that somatic sensation by acupuncture and visceral nociceptive stimulation can converge in the nucleus tractus solitarii where neurons integrate signals impacting on the function of organs. To explore the role of the nucleus tractus solitarii in the protective mechanism of pre-moxibustion on gastric mucosa, nucleus tractus solitarii were damaged in rats and pre-moxibustion treatment at the Zusanli (ST36) point followed. The gastric mucosa was then damaged by the anhydrous ethanol lavage method. Morphological observations, enzyme linked immunosorbent assays, and western immunoblot analyses showed that gastric mucosa surface lesion and the infiltration of inflammatory cells were significantly ameliorated after pre-moxibustion treatment. Furthermore, the gastric mucosal damage index and somatostatin level were reduced, and epidermal growth factor content in the gastric mucosa and heat-shock protein-70 expression were increased. These results were reversed by damage to the nucleus tractus solitarii. These findings suggest that moxibustion pretreatment at the Zusanli point is protective against acute gastric mucosa injury, and nucleus tractus solitarii damage inhibits these responses. Therefore, the nucleus tractus solitarii may be an important area for regulating the signal transduction of the protective effect of pre-moxibustion on gastric mucosa.