中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (6): 602-609.doi: 10.4103/1673-5374.130101

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

雷帕霉素促进许旺细胞迁移及神经生长因子的分泌

  

  • 收稿日期:2014-02-22 出版日期:2014-03-22 发布日期:2014-03-22
  • 基金资助:

    国家重点基础研究发展计划“973”子课题(2011CB606205),国家自然科学基金项目(51172171,51103112),教育部科学技术研究重大项目(313041), 湖北省自然科学基金项目(2013CFB354),中央高校基础研究项目(WUT: 2013-Ⅳ-099)

Rapamycin promotes Schwann cell migration and nerve growth factor secretion

Fang Liu 1, Haiwei Zhang 1, Kaiming Zhang 1, Xinyu Wang 2, Shipu Li 2, Yixia Yin 2   

  1. 1 Department of Orthopedics, Second Hospital of Yueyang, Yueyang, Hunan Province, China
    2 State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan, Hunan Province, China
  • Received:2014-02-22 Online:2014-03-22 Published:2014-03-22
  • Contact: Yixia Yin, Ph.D., State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan 430070, Hunan Province, China, yinyixia@whut.edu.cn.
  • Supported by:

    This work was supported by the Major State Basic Research Development Program of China (973 Program), No. 2011CB606205; the National Natural Science Foundation of China, No. 51172171 and 51103112; the Key Project of Chinese Ministry of Education, No. 313041; the Natural Science Foundation of Hubei Province, No. 2013CFB354; the Fundamental Research Funds for the Central Universities, No. WUT: 2013-IV-099.

摘要:

鉴于雷帕霉素与FK506相似,在体外也具有促进神经再生的作用。作者假设两者促进周围神经再生的作用机制相似。同时,实验拟通过对比不同浓度的雷帕霉素和FK506对许旺细胞的影响,探索雷帕霉素改善周围神经再生的作用效果与机制。结果发现最低浓度的雷帕霉素(1.53nmol/L)较最高浓度的FK506(100μmol/L) 能更加显著地促进许旺细胞迁移。其机制可能为雷帕霉素促进了许旺细胞神经生长因子的分泌和促进和生长相关蛋白43表达上调有关。实验过程中虽然雷帕霉素并未能显著影响许旺细胞的增殖,但也显示其具有良好的促周围神经再生的应用前景。

关键词: 神经再生, 周围神经损伤, 雷帕霉素, FK506, 许旺细胞, 细胞迁移, 神经生长因子, GAP43, NSFA grant

Abstract:

Rapamycin, similar to FK506, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of rapamycin and FK506 on Schwann cells and investigated effects and mechanisms of rapamycin on improving peripheral nerve regeneration. Results demonstrated that the lowest rapamycin concentration (1.53 nmol/L) more significantly promoted Schwann cell migration than the highest FK506 concentration (100 μmol/L). Rapamycin promoted the secretion of nerve growth factors and upregulated growth-associated protein 43 expression in Schwann cells, but did not significantly affect Schwann cell proliferation. Therefore, rapamycin has potential application in peripheral nerve regeneration therapy.

Key words: nerve regeneration, peripheral nerve injury, rapamycin, FK506, Schwann cell, cell migration, nerve growth factor, growth-associated protein 43, NSFC grant, neural regeneration