中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (6): 646-652.doi: 10.4103/1673-5374.130112

• 原著:退行性病与再生 • 上一篇    下一篇

米非司酮可调节5-羟色胺转运体的功能

  

  • 收稿日期:2014-02-24 出版日期:2014-03-22 发布日期:2014-03-22

Mifepristone modulates serotonin transporter function

Chaokun Li, Linlin Shan, Xinjuan Li, Linyu Wei, Dongliang Li   

  1. Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan Province, China
  • Received:2014-02-24 Online:2014-03-22 Published:2014-03-22
  • Contact: Chaokun Li, Ph.D., Department of Physiology and Neurobiology, Xinxiang Medical University, #601 Jinsui Road, Xinxiang 453003, Henan Province, China, lichaokun@hotmail.com.

摘要:

调节5-羟色胺的表达能够治疗精神病性抑郁症,糖皮质激素受体拮抗剂米非司酮作为治疗精神病性抑郁症的有效药物,其作用途径是否与5-羟色胺转运体的表达有关,答案尚不明确。实验将人脑5-羟色胺转运体克隆至非洲爪蟾卵母细胞中建立人脑5-羟色胺转运体的体外表达系统,使用双电极电压钳检测发现,米非司酮可直接通过抑制5-羟色胺转运体,减弱其活性,提示5-羟色胺转运体是米非司酮抗抑郁的药理学靶标。

关键词: 神经再生, 精神病性抑郁症, 米非司酮, 5-羟色胺转运体, 非洲爪蟾卵母细胞, 5-羟色胺, 抑郁症, 双电极电压钳, 电流记录, 抗抑郁药, 5-羟色胺转运, NSFC grant

Abstract:

Regulating serotonin expression can be used to treat psychotic depression. Mifepristone, a glucocorticoid receptor antagonist, is an effective candidate for psychotic depression treatment. However, the underlying mechanism related to serotonin transporter expression is poorly understood. In this study, we cloned the human brain serotonin transporter into Xenopus oocytes, to establish an in vitro expression system. Two-electrode voltage clamp recordings were used to detect serotonin transporter activity. Our results show that mifepristone attenuates serotonin transporter activity by directly inhibiting the serotonin transporter, and suggests that the serotonin transporter is a pharmacological target of mifepristone for the treatment of psychotic depression.

Key words: nerve regeneration, psychotic depression, mifepristone, serotonin transporter, Xenopus oocyte, serotonin, depression, two-electrode voltage clamp, current recording, antidepressants, serotonin transmission, NSFC grant, neural regeneration