中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (7): 741-748.doi: 10.4103/1673-5374.131580

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

复方中药侯氏黑散保护脑缺血神经血管单元的作用

  

  • 出版日期:2014-04-15 发布日期:2014-04-15
  • 基金资助:

    国家自然科学基金(No.30973782;No.81373526);北京市自然科学基金(No.7102014;No.7122018);北京市属高等学校人才强教深化计划“中青年骨干人才培养计划”项目(No.PXM2011014226)

Houshiheisan compound prescription protects neurovascular units after cerebral ischemia

Haizheng Wang, Lei Wang, Nan Zhang, Qi Zhang, Hui Zhao, Qiuxia Zhang   

  1. School of Traditional Chinese Medicine, Capital Medical University, Beijing, China
  • Online:2014-04-15 Published:2014-04-15
  • Contact: Qiuxia Zhang, M.D., School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China; Hui Zhao, M.D., School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China, zqx26@163.com; zhaohui8957@sina.com.

摘要:

复方中药侯氏黑散由风药(菊花、防风、细辛、桂枝、川芎、桔梗)和补虚药(人参、当归、白术、茯苓、干姜)组成。实验假设这些药对脑缺血神经血管单元有保护作用,分别用复方侯氏黑散 (风药+补虚药)及其中的风药和补虚药灌胃干预局灶性脑缺血大鼠模型。苏木精-伊红染色、透射电镜、免疫荧光染色及Western blot检测结果显示,侯氏黑散可减轻缺血大鼠脑缺血半暗带区的病理学损伤,保护神经血管单元,显著上调神经元核抗原NeuN的表达,下调淀粉样前体蛋白和β淀粉样蛋白42的表达,单用侯氏黑散方中风药或补虚药,虽对缺血半暗带神经元核抗原NeuN具有不同程度的保护作用,但对淀粉样前体蛋白和β淀粉样蛋白42的异常表达无影响。结果提示,复方侯氏黑散才能有效抑制脑缺血半暗带淀粉样前体蛋白异常积聚,减少淀粉样物质沉积,维持神经血管单元内环境的稳定性,减轻神经血管单元损伤。

关键词: 神经再生, 脑损伤, 脑缺血, 侯氏黑散, 风药, 补虚药, 神经血管单元, 淀粉样前体蛋白, β淀粉样蛋白, 神经元核抗原NeuN, 国家自然科学基金

Abstract:

Houshiheisan is composed of wind-dispelling (chrysanthemun flower, divaricate saposhnikovia root, Manchurian wild ginger, cassia twig, Szechwan lovage rhizome, and platycodon root) and deficiency-nourishing (ginseng, Chinese angelica, large-head atractylodes rhizome, Indian bread, and zingiber) drugs. In this study, we assumed these drugs have protective effects against cerebral ischemia, on neurovascular units. Houshiheisan was intragastrically administered in a rat model of focal cerebral ischemia. Hematoxylin-eosin staining, transmission electron microscopy, immunofluorescence staining, and western blot assays showed that Houshiheisan reduced pathological injury to the ischemic penumbra, protected neurovascular units, visibly up-regulated neuronal nuclear antigen expression, and down-regulated amyloid precursor protein and amyloid-β 42 expression. Wind-dispelling and deficiency-nourishing drugs maintained NeuN expression to varying degrees, but did not affect amyloid precursor protein or amyloid-β 42 expression in the ischemic penumbra. Our results suggest that the compound prescription Houshiheisan effectively suppresses abnormal amyloid precursor protein accumulation, reduces amyloid substance deposition, maintains stabilization of the internal environment of neurovascular units, and minimizes injury to neurovascular units in the ischemic penumbra.

Key words: nerve regeneration, brain injury, cerebral ischemia, Houshiheisan, wind-dispelling dru-gs, deficiency-nourishing drugs, neurovascular units, amyloid precursor protein, β-amyloid, neuronal nuclear antigen, NSFC grant, neural regeneration